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一种基于二氰基异佛尔酮骨架合理构建的荧光化学传感器，用于对铁和汞进行鉴别传感以及在HeLa细胞和斑马鱼中成像。

A reasonably constructed fluorescent chemosensor based on the dicyanoisophorone skeleton for the discriminative sensing of Fe and Hg as well as imaging in HeLa cells and zebrafish.

作者信息

Zhang Chuqi, Lv Xinyan, Liu Xiuhong, Chen Hongyun, He Haifeng

机构信息

School of Chemistry and Chemical Engineering, Jiangxi Science and Technology Normal University Nanchang 330013 People's Republic of China

出版信息

RSC Adv. 2022 Apr 25;12(20):12355-12362. doi: 10.1039/d2ra01357f. eCollection 2022 Apr 22.

DOI:10.1039/d2ra01357f
PMID:35480345
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9037825/
Abstract

In this study, a new fluorescent sensor dicyanoisophorone Rhodanine-3-acetic acid (DCI-RDA) (DCI-RDA) has been developed by employing a DCI-based push-pull dye as the fluorophore and RDA as the recognition moiety for the simultaneous sensing of Fe and Hg with a large Stokes Shift (162 nm), high selectivity and sensitivity, and low LOD (1.468 μM for Fe and 0.305 μM for Hg). In particular, DCI-RDA has a short response time (30 s). The Job's plot method in combination with H NMR titration and theoretical calculations was used to determine the stoichiometry of both DCI-RDA-Fe/Hg complexes to be 1 : 1. Moreover, DCI-RDA is applied as a fluorescent probe for imaging in HeLa cells and zebrafish, indicating that it can be potentially applied for Fe/Hg sensing in the field of biology.

摘要

在本研究中，通过使用基于二氰基异佛尔酮的推拉染料作为荧光团，罗丹宁-3-乙酸（RDA）作为识别部分，开发了一种新型荧光传感器二氰基异佛尔酮罗丹宁-3-乙酸（DCI-RDA），用于同时检测铁和汞，具有大斯托克斯位移（162 nm）、高选择性和灵敏度以及低检测限（铁为1.468 μM，汞为0.305 μM）。特别是，DCI-RDA具有短响应时间（30 s）。采用Job曲线法结合核磁共振氢谱滴定和理论计算确定DCI-RDA-铁/汞配合物的化学计量比均为1∶1。此外，DCI-RDA被用作HeLa细胞和斑马鱼成像的荧光探针，表明它有可能应用于生物学领域的铁/汞传感。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/338de148e6dd/d2ra01357f-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/12db13f42407/d2ra01357f-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/b0824d80cc3d/d2ra01357f-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/3631254fb22f/d2ra01357f-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/bb0d1e2a8645/d2ra01357f-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/4a05b6505b74/d2ra01357f-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/197fbfe4765a/d2ra01357f-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/a1d8065a8d79/d2ra01357f-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/302a521f1caa/d2ra01357f-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/338de148e6dd/d2ra01357f-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/12db13f42407/d2ra01357f-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/b0824d80cc3d/d2ra01357f-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/3631254fb22f/d2ra01357f-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/bb0d1e2a8645/d2ra01357f-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/4a05b6505b74/d2ra01357f-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/197fbfe4765a/d2ra01357f-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/a1d8065a8d79/d2ra01357f-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/302a521f1caa/d2ra01357f-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/9037825/338de148e6dd/d2ra01357f-f9.jpg

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