Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials，Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, People's Republic of China.
Biomed Mater. 2021 Jun 25;16(4). doi: 10.1088/1748-605X/ac08aa.
In this study, the silk fibroin/nano-hydroxyapatite/hyaluronic acid (SF/nHAp/HA) composite scaffolds with different HA contents were developed by blending, cross-linking and freeze-drying, and their physicochemical properties and cell biocompatibilitywere subsequently studied. It was observed that the molecular conformation of the composite scaffolds was mainly composed of silk I and a small amount of the-sheets structure. On enhancing the HA content, the pore size of the scaffold decreased, while the porosity, water absorption, swelling ratio and mechanical properties were observed to increase. In particular, the SF/nHAp/HA scaffold with a 5.0 wt% ratio exhibited the highest water absorption and mechanical properties among the developed materials. In addition, thecytocompatibility analysis showed that the bone marrow mesenchymal stem cells exhibited excellent cell proliferation and osteogenic differentiation ability on the SF/nHAp/5.0 wt%HA scaffolds, as compared with the other scaffolds. It can be concluded that the developed composite scaffolds represent a promising class of materials for the bone tissue repair and regeneration.
在这项研究中，通过混合、交联和冷冻干燥制备了不同 HA 含量的丝素蛋白/纳米羟基磷灰石/透明质酸（SF/nHAp/HA）复合支架，并研究了其物理化学性质和细胞生物相容性。结果表明，复合支架的分子构象主要由丝素 I 和少量的β-折叠结构组成。随着 HA 含量的增加，支架的孔径减小，而孔隙率、吸水率、溶胀率和力学性能则增加。特别是，在 5.0wt%比例下的 SF/nHAp/HA 支架具有最高的吸水率和力学性能。此外，细胞相容性分析表明，与其他支架相比，骨髓间充质干细胞在 SF/nHAp/5.0wt%HA 支架上表现出优异的细胞增殖和成骨分化能力。可以得出结论，所开发的复合支架是一种很有前途的用于骨组织修复和再生的材料。
