Heredia Fabiana, Volonté Yanel, Pereirinha Joana, Fernandez-Acosta Magdalena, Casimiro Andreia P, Belém Cláudia G, Viegas Filipe, Tanaka Kohtaro, Menezes Juliane, Arana Maite, Cardoso Gisele A, Macedo André, Kotowicz Malwina, Prado Spalm Facundo H, Dibo Marcos J, Monfardini Raquel D, Torres Tatiana T, Mendes César S, Garelli Andres, Gontijo Alisson M
CEDOC, Chronic Diseases Research Center, NOVA Medical School | Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Lisbon, Portugal.
INIBIBB, Instituto de Investigaciones Bioquímicas de Bahia Blanca, Universidad Nacional del Sur - CONICET, Bahía Blanca, Argentina.
Nat Commun. 2021 Jun 7;12(1):3328. doi: 10.1038/s41467-021-23218-5.
Innate behaviors consist of a succession of genetically-hardwired motor and physiological subprograms that can be coupled to drastic morphogenetic changes. How these integrative responses are orchestrated is not completely understood. Here, we provide insight into these mechanisms by studying pupariation, a multi-step innate behavior of Drosophila larvae that is critical for survival during metamorphosis. We find that the steroid-hormone ecdysone triggers parallel pupariation neuromotor and morphogenetic subprograms, which include the induction of the relaxin-peptide hormone, Dilp8, in the epidermis. Dilp8 acts on six Lgr3-positive thoracic interneurons to couple both subprograms in time and to instruct neuromotor subprogram switching during behavior. Our work reveals that interorgan feedback gates progression between subunits of an innate behavior and points to an ancestral neuromodulatory function of relaxin signaling.
先天行为由一系列基因编码的运动和生理子程序组成，这些子程序可与剧烈的形态发生变化相耦合。这些整合反应是如何协调的尚未完全了解。在这里，我们通过研究化蛹过程来深入了解这些机制，化蛹是果蝇幼虫的一种多步骤先天行为，对变态发育期间的生存至关重要。我们发现类固醇激素蜕皮激素触发平行的化蛹神经运动和形态发生子程序，其中包括在表皮中诱导松弛素肽激素Dilp8。Dilp8作用于六个Lgr3阳性胸中间神经元，以在时间上耦合这两个子程序，并在行为过程中指导神经运动子程序的转换。我们的研究揭示了器官间反馈控制着先天行为各亚单元之间的进程，并指出了松弛素信号传导的一种原始神经调节功能。
