Delgadillo R A, Smets R M, Yang Q R, Vanden Berghe D, Neetens A
Institute of Experimental Ophthalmology, University of Antwerp, Belgium.
Experientia. 1988 Aug 15;44(8):702-4. doi: 10.1007/BF01941034.
Sucrose, nycodenz, metrizamide and a mixture of equal volumes of sucrose and metrizamide were used as density gradient media for the isolation of retinal rod outer segments. The high osmolarity of sucrose had a strongly negative effect on the nature of the rod outer segments, whereas they were much better preserved using iodinated density gradient media such as nycodenz and metrizamide for their isolation.
蔗糖、尼可酰胺、甲泛葡胺以及等体积蔗糖与甲泛葡胺的混合物被用作密度梯度介质，用于分离视网膜视杆细胞外段。蔗糖的高渗透压对视杆细胞外段的性质有强烈的负面影响，而使用碘化密度梯度介质（如尼可酰胺和甲泛葡胺）进行分离时，视杆细胞外段能得到更好的保存。
