Colley N J, Hall M O
Exp Eye Res. 1986 Apr;42(4):323-9. doi: 10.1016/0014-4835(86)90025-4.
Rod outer segments (ROS) isolated from adult rat retinas are phagocytized by cultured rat retinal pigment epithelial (RPE) cells. Using a double immunofluorescent labeling procedure, we have compared the binding and ingestion of ROS isolated at different times of the day. After 2 hr of incubation, approximately 98% of the ROS are ingested, while 2% are still attached to the RPE cell surface, irrespective of the time of day or lighting conditions under which the ROS are isolated. These findings differ from those reported earlier, using a radioactive method for quantitating ROS phagocytosis (Hall, 1978).
从成年大鼠视网膜分离出的视杆细胞外段（ROS）可被培养的大鼠视网膜色素上皮（RPE）细胞吞噬。我们采用双重免疫荧光标记法，比较了在一天中不同时间分离出的ROS的结合和摄取情况。孵育2小时后，无论分离ROS时的时间或光照条件如何，约98%的ROS被摄取，而2%仍附着在RPE细胞表面。这些发现与早期使用放射性方法定量ROS吞噬作用的报道结果不同（霍尔，1978年）。
