人及小鼠基底膜蛋白BM - 40（骨连接蛋白、富含半胱氨酸的酸性分泌蛋白）的克隆及完整氨基酸序列
Cloning and complete amino acid sequences of human and murine basement membrane protein BM-40 (SPARC, osteonectin).
作者信息
Lankat-Buttgereit B, Mann K, Deutzmann R, Timpl R, Krieg T
机构信息
Dermatologische Klinik der Universität München, FRG.
出版信息
FEBS Lett. 1988 Aug 29;236(2):352-6. doi: 10.1016/0014-5793(88)80054-1.
Amino acid sequences of 285 and 286 residues, respectively, were deduced for mouse and human BM-40 from cDNA clones isolated from expression libraries. The sequences showed 92% identity and were also essentially identical to those of bone osteonectin and of the parietal endoderm protein SPARC. About 60% of the mouse BM-40 sequence was confirmed by Edman degradation. Two of the seven disulfide bonds were localized which apparently separate two distinct domains of mouse BM-40.
从表达文库中分离得到的小鼠和人BM - 40的cDNA克隆分别推导了285和286个残基的氨基酸序列。这些序列显示出92%的同一性，并且与骨骨连接蛋白和壁内胚层蛋白SPARC的序列基本相同。小鼠BM - 40序列的约60%通过埃德曼降解得到证实。确定了七个二硫键中的两个，它们明显分隔了小鼠BM - 40的两个不同结构域。
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