Kanitakis J, Zambruno G, Wang Y Z, Roche P, Berti E, Schmitt D
Laboratory of Dermatology, INSERM U346, Hôpital Edouard Herriot, Lyon, France.
Arch Dermatol Res. 1993;285(6):313-21. doi: 10.1007/BF00371830.
NU-T2 is a mouse monoclonal IgG1 antibody to the CD1b molecule, (cross-)reacting with an antigen of the dermal-epidermal junction (NU-T2 DEJ AG). Further immunohistochemical characterization of the NU-T2 DEJ AG showed it to display unique properties that differentiate it from other known antigens of the dermal-epidermal junction. Indeed, the NU-T2 DEJ AG is primate-specific and present only in epithelial basement membranes. In normal human skin it is expressed within the lowermost lamina lucida of the dermal-epidermal junction but not in the deep part of epidermal appendages nor in the deep part of epidermal appendages nor in the basement membrane of dermal vessels, smooth muscles or nerves. In diseases with intraepidermal or intradermal cleavage, NU-T2 reactivity was observed at the floor of the blister. In various skin specimens with a cleavage through the lamina lucida (NaCl--or dispase-split skin, bullous pemphigoid, junctional epidermolysis bullosa), NU-T2 immunoreactivity seemed reduced, being localized at the dermal side of the cleavage. These results suggest that the antigen recognized by NU-T2 is a novel component of the lamina lucida of the dermal-epidermal junction, that seems to be important for dermal-epidermal adhesion.
NU-T2是一种针对CD1b分子的小鼠单克隆IgG1抗体，与真皮-表皮交界处的一种抗原（NU-T2 DEJ AG）发生（交叉）反应。对NU-T2 DEJ AG的进一步免疫组织化学特征分析表明，它具有独特的性质，使其有别于真皮-表皮交界处的其他已知抗原。事实上，NU-T2 DEJ AG是灵长类动物特有的，仅存在于上皮基底膜中。在正常人类皮肤中，它表达于真皮-表皮交界处最下方的透明层内，但在表皮附属器深部或真皮血管、平滑肌或神经的基底膜中不表达。在表皮内或真皮内发生裂隙的疾病中，在水疱底部观察到NU-T2反应性。在各种通过透明层发生裂隙的皮肤标本（氯化钠或 dispase 分离的皮肤、大疱性类天疱疮、交界性大疱性表皮松解症）中，NU-T2免疫反应性似乎降低，定位于裂隙的真皮侧。这些结果表明，NU-T2识别的抗原是真皮-表皮交界处透明层中的一种新成分，似乎对真皮-表皮黏附很重要。
