Institute of Biomedical Photonics and Sensing, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, Shaanxi, China.
School of Food Equipment Engineering and Science, Xi'an Jiaotong University, Xi'an, 710049, Shaanxi, China.
Anal Bioanal Chem. 2021 Aug;413(19):4887-4894. doi: 10.1007/s00216-021-03451-z. Epub 2021 Jun 8.
Azodicarbonamide (ADA), as a dough conditioner food additive in flour, can be turned into toxic biurea and semicarbazide after high temperature processing. Hence, the using of ADA in food material should be strictly controlled, and the detection of ADA is very important for consumers' safety and health. Herein, a simple and fast colorimetric strategy has been developed for ADA detection based on the MnO nanosheets-3,3',5,5'-tetramethylbenzidine (TMB)-glutathione (GSH) as oxidative sensing system (MnO-TMB-GSH). Since the ADA can selectively react with GSH via oxidizing the sulfydryl (-SH) group of GSH to disulfide bond (S-S), which makes GSH unable to reduce MnO nanosheets and restore its oxidase-like activity. The absorbance changes of the TMB solution depended on ADA content. The MnO-TMB-GSH colorimetric platform can detect the ADA with a linear range of 10 μmol L (11.6 ppm) to 400 μmol L (464 ppm), and the limit of detection (LOD) is 3.3 μmol L (3.51 ppm). Some potential interferences in real sample were tested and did not affect the MnO-TMB-GSH colorimetric platform for ADA detection. Furthermore, the sensing platform was applied for detecting ADA in real flour sample with a recovery of 96%-105% (RSD < 5%). This colorimetric method can effectively and rapidly detect ADA additives in flour less than the prescribed standard (45 mg kg), which shows a great potential for visualization analysis and on-site detection of ADA in flour. A simple and fast colorimetric strategy has been developed for azodicarbonamide (ADA) detection based on the MnO nanosheets-3,3',5,5'-tetramethylbenzidine (TMB)-glutathione (GSH) as oxidative sensing system (MnO-TMB-GSH).
偶氮二甲酰胺（ADA）作为面粉中的面团调理剂食品添加剂，经高温加工后可转化为有毒的双氰胺和氨基脲。因此，ADA 在食品材料中的使用应严格控制，ADA 的检测对消费者的安全和健康非常重要。在此，基于 MnO 纳米片-3,3',5,5'-四甲基联苯胺（TMB）-谷胱甘肽（GSH）作为氧化传感体系（MnO-TMB-GSH），开发了一种用于 ADA 检测的简单快速比色策略。由于 ADA 可以通过氧化 GSH 的巯基（-SH）基团将 GSH 选择性地转化为二硫键（S-S），从而使 GSH 无法还原 MnO 纳米片并恢复其氧化酶样活性。TMB 溶液的吸光度变化取决于 ADA 含量。MnO-TMB-GSH 比色平台可以检测 ADA 的线性范围为 10 μmol L（11.6 ppm）至 400 μmol L（464 ppm），检测限（LOD）为 3.3 μmol L（3.51 ppm）。测试了真实样品中的一些潜在干扰物，它们不会影响 ADA 检测的 MnO-TMB-GSH 比色平台。此外，该传感平台还用于检测真实面粉样品中的 ADA，回收率为 96%-105%（RSD<5%）。该比色方法可有效快速检测低于规定标准（45 mg kg）的面粉中 ADA 添加剂，为面粉中 ADA 的可视化分析和现场检测提供了很大的潜力。
Zotero 插件