Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, People's Republic of China.
Mikrochim Acta. 2019 Jul 3;186(8):498. doi: 10.1007/s00604-019-3613-4.
A fluorimetric and colorimetric method is described for the determination of glutathione (GSH) and silver (I). It is based on the use of MnO nanosheets that were prepared by solution mixing and exfoliation. They display oxidase-mimicking activity and can catalyze the oxidation of o-phenylenediamine (OPD) to form yellow 2,3-diaminophenazine (DAP) with an absorption maximum at 410 nm. DAP also has a yellow fluorescence (with a peak at 560 nm). The MnO nanosheets can be rapidly reduced to Mn by GSH. This reduces the efficiency of the oxidase mimic MnO and causes a decrease in fluorescence and absorbance intensity. However, on addition of Ag, a complex is formed with GSH. It prevents the destruction of MnO nanosheets so that the enzyme mimicking activity is retained. A dual-method for the determination of GSH and Ag(I) was developed. It has excellent sensitivity for GSH with lower detection limits of 62 nM (fluorimetric) and 0.94 μM (colorimetric). The respective data for Ag(I) are 70 nM and 1.15 μM. The assay was successfully applied to the determination of GSH and Ag(I) in spiked serum samples. Graphical abstract Schematic presentation of a method for colorimetric and fluorometric determination of glutathione (GSH) and silver(I). MnO nanosheets are reduced to Mn(II) by GSH. This reduces the enzyme-mimicking activity of MnO nanosheets and causes a decrease in fluorescence and absorbance. On addition of Ag(I), the enzyme-like activity is increasingly retained. A decrease in fluorescence and absorbance is not observed any longer.
一种荧光和比色法测定谷胱甘肽（GSH）和银（I）的方法。它基于使用 MnO 纳米片，MnO 纳米片是通过溶液混合和剥离制备的。它们显示出氧化酶模拟活性，并可以催化邻苯二胺（OPD）的氧化，形成最大吸收波长为 410nm 的黄色 2,3-二氨基吩嗪（DAP）。DAP 也具有黄色荧光（峰值为 560nm）。GSH 可迅速将 MnO 纳米片还原为 Mn。这降低了氧化酶模拟 MnO 的效率，并导致荧光和吸光度强度降低。然而，当加入 Ag 时，会与 GSH 形成复合物。它防止 MnO 纳米片的破坏，从而保留酶模拟活性。建立了一种同时测定 GSH 和 Ag（I）的双方法。它对 GSH 具有出色的灵敏度，检测限分别为 62nM（荧光法）和 0.94μM（比色法）。Ag（I）的相应数据分别为 70nM 和 1.15μM。该测定法成功地应用于加标血清样品中 GSH 和 Ag（I）的测定。 图表摘要 比色法和荧光法测定谷胱甘肽（GSH）和银（I）的方法示意图。GSH 将 MnO 纳米片还原为 Mn(II)。这降低了 MnO 纳米片的酶模拟活性，并导致荧光和吸光度降低。加入 Ag(I)后，酶样活性得到越来越多的保留。不再观察到荧光和吸光度的降低。
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