Department of Biological Sciences, Inha University, Incheon, South Korea.
Geriatr Gerontol Int. 2021 Aug;21(8):725-731. doi: 10.1111/ggi.14204. Epub 2021 Jun 7.
We examined the underlying mechanisms associated with the longevity effects of Korean mistletoe extract (KME) in Drosophila melanogaster.
We measured the lifespan of sirtuin, chico and foxo mutant flies fed KME, the expression of the forkhead box O (FOXO) target genes and insulin-like peptide genes, and the localization of FOXO in flies fed the KME.
The longevity effect of KME was abolished in sirtuin, chico and foxo null mutant flies. In addition, the expression of FOXO target genes and the localization of FOXO into nuclei were increased in flies fed KME, but the expression of the insulin-like peptide genes was decreased by KME supplementation.
The results show that KME extends the fly lifespan through sirtuin-induced FOXO activation. We suggest that KME has potential use as a beneficial anti-aging and longevity supplement. Geriatr Gerontol Int 2021; 21: 725-731.
我们研究了朝鲜槲寄生提取物（KME）对黑腹果蝇寿命的影响的潜在机制。
我们测量了喂食 KME 的 sir2、chico 和 foxo 基因突变体果蝇的寿命、叉头框 O（FOXO）靶基因和胰岛素样肽基因的表达以及 KME 喂养的果蝇中 FOXO 的定位。
KME 的长寿作用在 sir2、chico 和 foxo 缺失突变体果蝇中被消除。此外，FOXO 靶基因的表达和 FOXO 向核内的定位在喂食 KME 的果蝇中增加，但胰岛素样肽基因的表达被 KME 补充所抑制。
结果表明，KME 通过 sir2 诱导的 FOXO 激活延长了果蝇的寿命。我们认为 KME 有作为一种有益的抗衰老和长寿补充剂的潜力。老年医学与老年病学国际 2021；21：725-731。
