巴西氟喹诺酮类耐药尿路致病性大肠埃希菌的基因组分析。
Genome profiling of fluoroquinolone-resistant uropathogenic Escherichia coli isolates from Brazil.
机构信息
Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.
Department of Chemical and Biological Sciences, Institute of Biosciences, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
出版信息
Braz J Microbiol. 2021 Sep;52(3):1067-1075. doi: 10.1007/s42770-021-00513-3. Epub 2021 Jun 8.
Urinary tract infections (UTIs) are a major public health concern in both community and hospital settings worldwide. Uropathogenic Escherichia coli (UPEC) is the main causative agent of UTI and increasingly associated with antibiotic resistance. Herein, we report the draft genome sequence of 9 fluoroquinolone-resistant UPEC isolates from Brazil and examine selected major phenotypic features, such as antimicrobial resistance profile, phylogroup, serotype, sequence type (ST), virulence genes, and resistance marks. Besides the quinolone resistance, beta-lactams, ESBL production, aminoglycosides, and tetracycline resistance were observed. High prevalence of 20 virulence genes was detected in all isolates, such as those encoding type 1 fimbriae, acid tolerance system, and hemolysin E, particularly within E. coli B2 phylogroup, as ST131 and ST1193 strains, among other genomic analyses as genomic islands, resistance plasmids, and integron identification.
尿路感染（UTIs）是全球社区和医院环境中的一个主要公共卫生问题。尿路致病性大肠杆菌（UPEC）是 UTI 的主要病原体，并且越来越多地与抗生素耐药性相关。在此，我们报告了来自巴西的 9 株氟喹诺酮耐药 UPEC 分离株的基因组草图序列，并研究了一些主要的表型特征，如抗菌药物耐药谱、 phylogroup、血清型、序列型（ST）、毒力基因和耐药标记。除了喹诺酮耐药性外，还观察到了β-内酰胺类、ESBL 产生、氨基糖苷类和四环素耐药性。所有分离株均检测到 20 种毒力基因的高流行率，例如编码 1 型菌毛、耐酸系统和溶血素 E 的基因，特别是在大肠杆菌 B2 phylogroup 中，如 ST131 和 ST1193 菌株等，还进行了基因组岛、耐药质粒和整合子鉴定等其他基因组分析。
Zotero 插件