Department of Obstetrics, Maternal and child health hospital of Hunan Province Changsha, Hunan, China.
Department of Hospital office, Maternal and child health hospital of Hunan Province Changsha, Hunan, China.
Ginekol Pol. 2024;95(9):711-717. doi: 10.5603/GP.a2021.0126. Epub 2021 Jun 9.
Preeclampsia is one of the most feared complications of pregnancy, which can progress rapidly to serious complications such as death of both mother and fetus. To present, the leading cause of preeclampsia is still debated. The purpose of this article was to explore the clinical significance of S100B protein, a kind of Ca2+ -sensor protein, in the early-onset severe preeclampsia.
Nine pregnant women with early-onset severe preeclampsia (the study group) and 13 healthy pregnant women (the control group) were included in this study. The level of S100B in the amniotic fluid, maternal blood, and umbilical cord blood were detected by enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance imaging (SPRi) methods. Diagnostic values of S100B for early-onset severe preeclampsia were assessed by Receiver Operating Characteristic (ROC) curve analysis.
The levels of S100B in maternal blood and amniotic fluid in the study group were higher than those in the control group (p < 0.05). ROC curve analysis showed that S100B detected by SPRi method (SPRi-S100B) showed a cut-off level of 181 ng/mL with sensitivity of 100%, a specificity of 84.6%, and a Youden index of 0.846 in the maternal blood, which had better clinical significance and diagnostic value (at than that detected by ELISA (ELISA-S100B).
The levels of S100B detected by SPRi in maternal blood can indicate early-onset severe preeclampsia and perinatal brain injury.
子痫前期是妊娠最可怕的并发症之一，可迅速进展为严重并发症，如母婴死亡。目前，子痫前期的主要病因仍存在争议。本文旨在探讨钙传感器蛋白 S100B 在早发型重度子痫前期中的临床意义。
本研究纳入了 9 例早发型重度子痫前期孕妇（研究组）和 13 例健康孕妇（对照组）。采用酶联免疫吸附试验（ELISA）和表面等离子体共振成像（SPRi）方法检测羊水中、母血中和脐血中 S100B 蛋白的水平。通过受试者工作特征（ROC）曲线分析评估 S100B 蛋白对早发型重度子痫前期的诊断价值。
研究组母血和羊水 S100B 蛋白水平均高于对照组（p＜0.05）。ROC 曲线分析显示，SPRi 法检测的 S100B 蛋白（SPRi-S100B）在母血中的截断值为 181ng/ml，其灵敏度为 100%，特异性为 84.6%，Youden 指数为 0.846，具有更好的临床意义和诊断价值（优于 ELISA 法检测的 S100B 蛋白（ELISA-S100B）。
SPRi 法检测母血中 S100B 蛋白水平可提示早发型重度子痫前期及围生期脑损伤。
