Engels W, Kamps M A, Lemmens P J, van der Vusse G J, Reneman R S
Department of Medical Microbiology, University of Limburg, Maastricht, The Netherlands.
J Chromatogr. 1988 Jun 3;427(2):209-18. doi: 10.1016/0378-4347(88)80123-3.
A highly sensitive and specific assay for the quantitation of prostaglandins (PGs) such as PGE1, PGE2, PGF1 alpha, PGF2 alpha, 6-keto-PGF1 alpha, and including thromboxane B2, is described. The method involves the addition of PGF1 alpha and PGE1 as the internal standards, extraction from whole blood and purification by silica gel column chromatography. Following conversion into the methoximes, purification by reversed-phase chromatography and esterification with panacyl bromide, samples are analysed by high-performance liquid chromatography with fluorimetric detection. The lower limit of detection of the eicosanoids 6-keto-PGF1 alpha, thromboxane B2 and PGF2 alpha in blood is ca. 50 pg/ml and that of PGE2 is 100 pg/ml. Assay linearity is demonstrated over a range from 60 pg to 60 ng of eicosanoid injected. The method allows simultaneous assessment of prostaglandins and thromboxane extracted from complex biological fluids at picogram levels.
本文描述了一种用于定量前列腺素（PGs）的高灵敏度和特异性分析方法，这些前列腺素包括PGE1、PGE2、PGF1α、PGF2α、6-酮-PGF1α，以及血栓素B2。该方法包括添加PGF1α和PGE1作为内标，从全血中提取并通过硅胶柱色谱法进行纯化。在转化为甲氧肟后，通过反相色谱法纯化并用泛酰溴酯化，然后通过高效液相色谱法和荧光检测对样品进行分析。血液中类花生酸6-酮-PGF1α、血栓素B2和PGF2α的检测下限约为50 pg/ml，PGE2的检测下限为100 pg/ml。在注入60 pg至60 ng类花生酸的范围内证明了分析的线性。该方法能够同时评估从复杂生物流体中提取的皮克水平的前列腺素和血栓素。
