The Westmead Institute for Medical Research, University of Sydney, Sydney, Australia.
Marie Bashir Institute for Infectious Diseases and Biosecurity, University of Sydney, Sydney, Australia.
Microb Genom. 2021 Jun;7(6). doi: 10.1099/mgen.0.000554.
Enterotoxigenic (ETEC) expressing the colonization pili CFA/I are common causes of diarrhoeal infections in humans. Here, we use a combination of transposon mutagenesis and transcriptomic analysis to identify genes and pathways that contribute to ETEC persistence in water environments and colonization of a mammalian host. ETEC persisting in water exhibit a distinct RNA expression profile from those growing in richer media. Multiple pathways were identified that contribute to water survival, including lipopolysaccharide biosynthesis and stress response regulons. The analysis also indicated that ETEC growing in mice encounter a bottleneck driving down the diversity of colonizing ETEC populations.
产肠毒素性（ETEC）表达定植菌毛 CFA/I 是人类腹泻感染的常见原因。在这里，我们使用转座子诱变和转录组分析的组合来鉴定有助于 ETEC 在水环境中存活和在哺乳动物宿主中定植的基因和途径。在水中持续存在的 ETEC 与在更丰富的培养基中生长的 ETEC 表现出明显不同的 RNA 表达谱。确定了多个有助于水生存的途径，包括脂多糖生物合成和应激反应调节子。该分析还表明，在小鼠中生长的 ETEC 遇到了一个瓶颈，降低了定植 ETEC 种群的多样性。
