Homi Bhabha National Institute, Training School Complex, Anushaktinagar, Mumbai, 400094, India.
Laser Biomedical Applications Division, Raja Ramanna Centre of Advanced Technology, Indore, 452013, India.
Eur Biophys J. 2021 Sep;50(6):867-876. doi: 10.1007/s00249-021-01541-5. Epub 2021 Jun 10.
Management of postprandial hyperglycemia is important for preventing severe complications like cardiovascular disease in diabetes patients. The associated glycemic instability in postprandial hyperglycemia may also cause disorders in circulating red blood cells (RBCs). Therefore, effects of short-term hyperglycemic stress on RBCs such as occur in the postprandial condition, have been studied here ex vivo using single-cell Raman spectroscopy and optical trapping. RBCs incubated in high glucose containing media relevant to postprandial hyperglycemia were studied for changes with respect to controls by analyzing the single-cell Raman spectra acquired with Raman optical tweezers with 532 nm excitation light. Use of 532 nm light for exciting Raman spectra also results in simultaneous photoreduction of intracellular hemoglobin (Hb). The level of photoreduction was noticed to be limited in hyperglycemia-exposed cells in comparison to the control. Since this suggests formation of permanently oxidized Hb in hyperglycemia-exposed RBCs, a fluorescence study was performed which showed elevated levels of oxidative stress in these cells. The changes in the RBC membrane, which may result due to higher levels of oxidative stress, were investigated using optical stretching experiments under the laser trap. The results indicated a loss of elasticity for the RBC membrane due to hyperglycemic exposure.
餐后高血糖的管理对于预防糖尿病患者的心血管等严重并发症非常重要。餐后高血糖相关的血糖不稳定也可能导致循环红细胞（RBC）紊乱。因此，本研究采用单细胞拉曼光谱和光阱技术，在体外用单细胞拉曼光谱法研究了餐后条件下红细胞的短期高血糖应激对 RBC 的影响。通过分析用 532nm 激发光的拉曼光镊获得的单细胞拉曼光谱，研究了在含有与餐后高血糖相关的高葡萄糖培养基中孵育的 RBC 相对于对照的变化。用 532nm 光激发拉曼光谱也会导致细胞内血红蛋白（Hb）的同时光还原。与对照相比，发现在高糖暴露的细胞中光还原的程度受到限制。由于这表明在高糖暴露的 RBC 中形成了永久性氧化 Hb，因此进行了荧光研究，显示这些细胞中的氧化应激水平升高。由于氧化应激水平较高，可能导致 RBC 膜发生变化，使用激光阱下的光学拉伸实验对其进行了研究。结果表明，由于高血糖暴露，RBC 膜的弹性丧失。
