Jia Wenguang, Chen Ping, Chen Wenqiang, Li Yongqing
Department of Pediatrics.
Guangxi Key Laboratory of Thalassemia Research, The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China.
Medicine (Baltimore). 2018 Sep;97(39):e12611. doi: 10.1097/MD.0000000000012611.
This study aimed to study the differences in Raman spectra of red blood cells (RBCs) among patients with β-thalassemia and controls using laser tweezers Raman spectroscopy (LTRS) system.A total of 33 patients with β-thalassemia major, 49 with β-thalassemia minor, and 65 controls were studied. Raman spectra of RBCs for each sample were recorded. Principal component analysis (PCA), one-way analysis of variance (ANOVA), and independent-sample t test were performed.The intensities of Raman spectra of β-thalassemia (major and minor) RBCs were lower than those of controls, especially at bands 1546, 1603, and 1619 cm. The intensity ratio of band 1546 cm to band 1448 cm demonstrated that there was a significant difference between the spectra of β-thalassemia major (mostly below 2.15) and those of controls. The spectra of controls could be well distinguished from those of β-thalassemia major using PCA. After normalization, the spectra of two different genotypes with β/β mutations mainly overlapped, while those with β/β mutations had lower intensity at bands 1546, 1603, and 1619 cm.The present study provided Raman characteristics of RBCs in patients with β-thalassemia major and supported the use of LTRS as a method for screening β-thalassemia major. The recognition rate for β-thalassemia minor needs to be further improved.
本研究旨在利用激光镊子拉曼光谱（LTRS）系统研究β地中海贫血患者与对照组红细胞（RBC）的拉曼光谱差异。共研究了33例重型β地中海贫血患者、49例轻型β地中海贫血患者和65例对照者。记录了每个样本红细胞的拉曼光谱。进行了主成分分析（PCA）、单因素方差分析（ANOVA）和独立样本t检验。重型和轻型β地中海贫血患者红细胞拉曼光谱的强度低于对照组，尤其是在1546、1603和1619cm波段。1546cm波段与1448cm波段的强度比表明，重型β地中海贫血患者（大多低于2.15）与对照组的光谱存在显著差异。使用PCA可以很好地区分对照组与重型β地中海贫血患者的光谱。归一化后，两种不同β/β突变基因型的光谱主要重叠，而β/β突变基因型在1546、1603和1619cm波段的强度较低。本研究提供了重型β地中海贫血患者红细胞的拉曼特征，并支持将LTRS作为重型β地中海贫血的筛查方法。轻型β地中海贫血的识别率有待进一步提高。
