Division of General Surgery, Peking University First Hospital, Beijing, China.
Central laboratory, Peking University First Hospital, Beijing, China.
FASEB J. 2021 Jul;35(7):e21720. doi: 10.1096/fj.202100266R.
Methylation of circulating free DNA (CfDNA) has emerged as an efficient marker of tumor screening and prognostics. However, no efficient methylation marker has been developed for monitoring liver metastasis (LM) in colorectal cancer (CRC). Utilizing methylome profiling and bisulfite sequencing polymerase chain reaction of paired primary and LM sites, significantly increased methylation of TCHH was identified in the process of LM in CRC in the present study. Methylight analysis of TCHH methylation in CfDNA displayed a promisingly discriminative power between CRC with and without LM. Besides, significant coefficient of TCHH methylation and LM tumor volume was also validated. Together, these results indicated the potential of TCHH methylation in CfDNA as a monitoring marker of LM in CRC.
循环游离 DNA（CfDNA）的甲基化已成为肿瘤筛查和预后的有效标志物。然而，目前尚未开发出用于监测结直肠癌（CRC）肝转移（LM）的有效甲基化标志物。本研究利用甲基化组谱分析和配对原发和 LM 部位的亚硫酸氢盐测序聚合酶链反应，发现 TCHH 在 CRC 的 LM 过程中显著增加了甲基化。CfDNA 中 TCHH 甲基化的 Methylight 分析显示了区分有和无 LM 的 CRC 的有希望的区分能力。此外，还验证了 TCHH 甲基化和 LM 肿瘤体积的显著系数。总之，这些结果表明 CfDNA 中 TCHH 甲基化作为 CRC LM 监测标志物的潜力。
