College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China.
College of Plant Protection, Gansu Agricultural University, Lanzhou, Gansu, China.
PLoS One. 2021 Jun 10;16(6):e0252865. doi: 10.1371/journal.pone.0252865. eCollection 2021.
Apple scab caused by Venturia inaequalis is a serious disease of cultivated apple worldwide. In this study, we collected 132 V. inaequalis isolates from Shaanxi, Gansu, Xinjiang, and the U.K. and analyzed their genetic diversity by using 13 microsatellite markers. Cluster analysis based on population structure and genetic distances suggested high similarity among the four regions. Population differentiation values ranged from 0.044 to 0.155, indicating there is a high level of kinship among the four regions. All isolates could be divided into 5 lineages with a 0.76 similarity coefficient. Among the four regions, Shaanxi had only one lineage, Group II; Gansu had four lineages, Group I, Group II, Group IV, and Group V; Xinjiang had all five lineages, Group I, Group II, Group III, Group IV, and Group V; and the U.K. had three lineages, Group I, Group II and Group IV. High molecular variance was detected for populations in the four regions, with 91% of the variance occurring within the populations and 9% among the populations. Structure analysis there are three common ancestors of these four regions. The results of the present study shed light on the genetic diversity of V. inaequalis in Shaanxi, Gansu and Xinjiang, which will lead to the development of more effective management strategies and new resistant apple cultivars through molecular marker-assisted selection.
由仁果外囊菌引起的苹果黑星病是一种严重的世界性苹果病害。本研究收集了来自陕西、甘肃、新疆和英国的 132 个仁果外囊菌分离株，并用 13 个微卫星标记分析了它们的遗传多样性。基于种群结构和遗传距离的聚类分析表明，四个地区之间具有高度相似性。种群分化值范围为 0.044 至 0.155，表明四个地区之间具有高度的亲缘关系。所有分离株可分为 5 个谱系，相似度系数为 0.76。四个地区中，陕西只有一个谱系，第二组；甘肃有四个谱系，第一组、第二组、第四组和第五组；新疆有所有五个谱系，第一组、第二组、第三组、第四组和第五组；英国有三个谱系，第一组、第二组和第四组。四个地区的种群均检测到高分子方差，91%的方差存在于种群内，9%的方差存在于种群间。结构分析表明，这四个地区存在三个共同的祖先。本研究结果阐明了陕西、甘肃和新疆仁果外囊菌的遗传多样性，这将通过分子标记辅助选择开发出更有效的管理策略和新的抗苹果品种。
