Dar Mohammad Saleem, Ahmad Mushtaq, Yetoo Nakeeb-Un-Nisa, Bhatt Bhagyshree, Bhat Suhail Nazir, Altaf Heena, Rafiqee Sumira, Nabi Asha, Mohiddin F A, Gaafar Abdel-Rhman Z, Mansoor Sheikh, Shah Mehraj D, Mushtaq Muntazir
Division of Plant Pathology, Faculty of Agriculture, SKUAST-Kashmir, Wadura, Sopore, Jammu and Kashmir 193201 India.
Division of Plant Pathology, Faculty of Horticulture, SKUAST-Kashmir, Shalimar, Srinagar, Jammu and Kashmir 190025 India.
3 Biotech. 2023 Aug;13(8):273. doi: 10.1007/s13205-023-03672-2. Epub 2023 Jul 12.
Apple scab instigated by impels remarkable losses to apple fruit production. In an effort to comprehend the key mechanisms of evolutionary potential defining population, 132 isolates of from five commercial apple orchards were collected and assayed using 14 microsatellite markers. The average diversity was observed within the individuals of populations based on the Shannon-Wieners index (I) and observed heterozygosity (Ho) was average but considerably lower than expected heterozygosity (He). The genetic differentiation based on FST values was revealed as an average measure of divergence between populations and had varying proportions of gene flow and migration among themselves. Analysis of Molecular Variance (AMOVA) revealed that variance (94%) was dispersed across individuals with a significant (6%) variation between populations from different regions. To examine host specialization within the . population, the assignment approach based on K-means of clustering (an unsupervised machine learning approach), revealed that the clustering method supported three clusters at ( = 3) and three major clusters were also observed in Principle Component Analysis (PCA). Additionally, Nei's genetic distance values, pairwise estimates of genetic differentiation, dendrogram using the neighbor-joining and PCoA revealed the random distribution of . isolates that depicted a high proportion of genotypic diversity within populations and population genetic structure.
The online version contains supplementary material available at 10.1007/s13205-023-03672-2.
由……引发的苹果黑星病给苹果果实生产造成了巨大损失。为了理解决定种群进化潜力的关键机制，从五个商业苹果园中收集了132个……分离株，并使用14个微卫星标记进行分析。基于香农 - 维纳指数（I）观察到种群个体内的平均多样性，观察杂合度（Ho）为平均水平，但远低于预期杂合度（He）。基于FST值的遗传分化被揭示为种群间差异的平均度量，它们之间的基因流和迁移比例各不相同。分子方差分析（AMOVA）表明，方差（94%）分散在个体中，不同区域的种群间存在显著的（6%）变异。为了研究……种群内的寄主专化性，基于K均值聚类的分配方法（一种无监督机器学习方法）表明，聚类方法在（ = 3）时支持三个聚类，主成分分析（PCA）中也观察到三个主要聚类。此外，内氏遗传距离值、成对遗传分化估计、使用邻接法的系统发育树和主坐标分析（PCoA）揭示了……分离株的随机分布，这表明种群内存在高比例的基因型多样性和种群遗传结构。
在线版本包含可在10.1007/s13205 - 023 - 03672 - 2获取的补充材料。 （注：原文中部分关键内容缺失具体信息，已按原样翻译）
