State Key Laboratory of Reliability and Intelligence of Electrical Equipment, Hebei University of Technology, Tianjin, 300130, PR China; Tianjin Key Laboratory of Bioelectromagnetic Technology and Intelligent Health, Hebei University of Technology, Tianjin, 300130, PR China.
State Key Laboratory of Reliability and Intelligence of Electrical Equipment, Hebei University of Technology, Tianjin, 300130, PR China; Tianjin Key Laboratory of Bioelectromagnetic Technology and Intelligent Health, Hebei University of Technology, Tianjin, 300130, PR China.
Neurosci Res. 2021 Dec;173:90-98. doi: 10.1016/j.neures.2021.05.015. Epub 2021 Jun 7.
This study primarily explored the potential effects of high-frequency (20 Hz) repetitive transcranial magnetic stimulation (rTMS) with different intervention protocols on cognition and neuronal excitability in mice. Mice were randomly divided into 4 groups: a control group that received sham stimulation, an rTMS in vitro group whose acute brain slices received high-frequency stimulation, an rTMS 1 d group that received high-frequency stimulation for only 1 d, and an rTMS 15 d group that received high-frequency stimulation for 15 d. The novel object recognition and step-down tests were used to assess cognitive ability. The patch-clamp technique was used to record the membrane potentials and neural discharges of dentate gyrus granule cells to evaluate neuronal excitability. Results revealed that cognition and neuronal excitability in the rTMS 15 d group were significantly increased than that in the control and rTMS 1 d groups. The neuronal excitability in the rTMS in vitro group was also significantly increased than that in the control and rTMS 1 d groups. No significant changes were observed between the control and rTMS 1 d groups. These results suggested that high-frequency rTMS applied to the acute brain slices of mice in vitro exerted an immediate effect on increasing neuronal excitability. Chronic high-frequency rTMS applied to the brain of mice in vivo exerted a cumulative effect in improving cognition and increasing neuronal excitability.
这项研究主要探讨了不同干预方案的高频（20Hz）重复经颅磁刺激（rTMS）对认知和神经元兴奋性的潜在影响。将小鼠随机分为 4 组：对照组接受假刺激，体外 rTMS 组急性脑片接受高频刺激，rTMS 1d 组仅接受 1d 高频刺激，rTMS 15d 组接受 15d 高频刺激。采用新物体识别和下台阶测试评估认知能力。应用膜片钳技术记录齿状回颗粒细胞的膜电位和神经放电，评估神经元兴奋性。结果表明，rTMS 15d 组的认知和神经元兴奋性明显高于对照组和 rTMS 1d 组。体外 rTMS 组的神经元兴奋性也明显高于对照组和 rTMS 1d 组。对照组和 rTMS 1d 组之间无显著变化。这些结果提示，高频 rTMS 作用于体外培养的小鼠急性脑片即刻增加神经元兴奋性，体内慢性高频 rTMS 则具有改善认知和增加神经元兴奋性的累积效应。
