Zhu Haijun, Yin Xiaonan, Yang Huilan, Fu Rui, Hou Wentao, Ding Chong, Xu Guizhi
Key Laboratory of Digital Medical Engineering of Hebei Province, College of Electronic and Information Engineering, Hebei University, Baoding, 071002 China.
State Key Laboratory of Reliability and Intelligence of Electrical Equipment, School of Electrical Engineering, Hebei University of Technology, Tianjin, 300130 China.
Cogn Neurodyn. 2023 Apr;17(2):431-443. doi: 10.1007/s11571-022-09837-8. Epub 2022 Jul 16.
This study aims to explore the effects of acute high-frequency repetitive transcranial magnetic stimulation (hf-rTMS) on neuronal excitability of granule cells in the hippocampal dentate gyrus, as well as the underlying intrinsic mediating mechanisms by which rTMS regulates neuronal excitability. First, high-frequency single TMS was used to measure the motor threshold (MT) of mice. Then, rTMS with different intensities of 0 MT (control), 0.8 MT, and 1.2 MT were applied to acute mice brain slices. Next, patch-clamp technique was used to record the resting membrane potential and evoked nerve discharge of granule cells, as well as the voltage-gated sodium current ( ) of voltage-gated sodium channels (VGSCs), transient outward potassium current ( ) and delayed rectifier potassium current ( ) of voltage-gated potassium channels (Kv). Results showed that acute hf-rTMS in both 0.8 MT and 1.2 MT groups significantly activated and inhibited and compared with control group, due to the changes of dynamic characteristics of VGSCs and Kv. Acute hf-rTMS in both 0.8 MT and 1.2 MT groups significantly increased membrane potential and nerve discharge frequency. Therefore, changing dynamic characteristics of VGSCs and Kv, activating and inhibiting and might be one of the intrinsic mediating mechanisms by which rTMS enhanced the neuronal excitability of granular cells, and this regulatory effect increased with the increase of stimulus intensity.
本研究旨在探讨急性高频重复经颅磁刺激（hf-rTMS）对海马齿状回颗粒细胞神经元兴奋性的影响，以及rTMS调节神经元兴奋性的潜在内在介导机制。首先，使用高频单次TMS测量小鼠的运动阈值（MT）。然后，将不同强度（0 MT（对照）、0.8 MT和1.2 MT）的rTMS应用于急性小鼠脑片。接下来，采用膜片钳技术记录颗粒细胞的静息膜电位和诱发神经放电，以及电压门控钠通道（VGSCs）的电压门控钠电流（ ）、电压门控钾通道（Kv）的瞬时外向钾电流（ ）和延迟整流钾电流（ ）。结果显示，与对照组相比，0.8 MT和1.2 MT组的急性hf-rTMS均显著激活了 并抑制了 和 ，这是由于VGSCs和Kv动态特性的改变所致。0.8 MT和1.2 MT组的急性hf-rTMS均显著增加了膜电位和神经放电频率。因此，改变VGSCs和Kv的动态特性，激活 并抑制 和 可能是rTMS增强颗粒细胞神经元兴奋性的内在介导机制之一，且这种调节作用随刺激强度的增加而增强。
