Robke Rhiannon, Hashemi Parastoo, Ramsson Eric
Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC, USA.
Department of Biomedical Science, Grand Valley State University, Allendale, MI, USA.
HardwareX. 2019 Apr;5. doi: 10.1016/j.ohx.2018.e00051. Epub 2018 Dec 31.
Fast-scan cyclic voltammetry (FSCV) is an analytical tool used to probe neurochemical processes in real-time. A major drawback for specialized applications of FSCV is that instrumentation must be constructed or modified in-house by those with expertise in electronics. One such specialized application is the newly developed fast-scan controlled-adsorption voltammetry (FSCAV) that measures basal (tonic) dopamine and serotonin concentrations. FSCAV requires additional software and equipment (an operational amplifier coupled to a transistor-transistor logic) allowing the system to switch between applying a FSCV waveform and a constant potential to the working electrode. Herein we describe a novel, simplified switching component to facilitate the integration of FSCAV into existing FSCV instruments, thereby making this method more accessible to the community. Specifically, we employ two light emitting diodes (LEDs) to generate the voltage needed to drive a NPN bipolar junction transistor, substantially streamlining the circuitry and fabrication of the switching component. We performed and analyses to compare the new LED circuit vs. the original switch. Our data shows that the novel simplified switching component performs equally well when compared to traditional instrumentation. Thus, we present a new, simplified scheme to perform FSCAV that is cheap, simple, and easy to construct by individuals without a background in engineering and electronics.
快速扫描循环伏安法(FSCV)是一种用于实时探测神经化学过程的分析工具。FSCV专门应用的一个主要缺点是,仪器必须由具有电子专业知识的人员在内部构建或修改。一种这样的专门应用是新开发的快速扫描控制吸附伏安法(FSCAV),它可以测量基础(张力性)多巴胺和血清素浓度。FSCAV需要额外的软件和设备(一个与晶体管-晶体管逻辑耦合的运算放大器),使系统能够在向工作电极施加FSCV波形和恒定电位之间切换。在此,我们描述了一种新颖、简化的切换组件,以促进FSCAV集成到现有的FSCV仪器中,从而使该方法更易于被社区使用。具体来说,我们使用两个发光二极管(LED)来产生驱动NPN双极结型晶体管所需的电压,大大简化了切换组件的电路和制造。我们进行了[具体分析内容缺失]分析,以比较新的LED电路与原始开关。我们的数据表明,与传统仪器相比,这种新颖的简化切换组件表现同样出色。因此,我们提出了一种执行FSCAV的新的简化方案,该方案成本低廉、简单,并且没有工程和电子背景的个人也易于构建。
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