Department of Food Science and Engineering, College of Agriculture, Yanbian University, Yanji 133000, Jilin, China.
Food Funct. 2021 Jul 21;12(14):6254-6270. doi: 10.1039/d1fo00907a. Epub 2021 Jun 11.
This study investigated lipid metabolism regulation by anthocyanins from Aronia melanocarpa (AAM) in 3T3-L1 preadipocytes and high fat diet (HFD) mice. Ultra-performance liquid chromatography/ion mobility quadrupole time-of-flight mass spectrometry analysis identified the constituents of AAM, which decreased lipid content and inflammation in 3T3-L1 cells without cytotoxicity. Meanwhile, taking normal diet and orlistat mice as references, AAM supplementation improved blood lipid levels and adipocyte degeneration, promoted beneficial gut microbial growth, and maintained lipid metabolism in HFD mice. Furthermore, AAM activated the AMP-activated protein kinase (AMPK) signaling pathway, accompanied by the regulation of adipogenic transcription factors and their target genes in vitro and in vivo. Collectively, our data demonstrated that AAM exhibits anti-adipogenic activities that were partially mediated by the AMPK pathway and gut microbiota regulation. This study provides new insight into the regulation of lipid metabolism by AAM and suggests that AAM has potential therapeutic effects on hyperlipidemia.
本研究探讨了黑果腺肋花楸（AAM）花色苷对 3T3-L1 前体脂肪细胞和高脂饮食（HFD）小鼠的脂代谢调节作用。超高效液相色谱/离子淌度四极杆飞行时间质谱分析鉴定了 AAM 的成分，其可降低 3T3-L1 细胞中的脂质含量和炎症，且无细胞毒性。同时，以正常饮食和奥利司他小鼠作为参考，AAM 补充可改善血脂水平和脂肪细胞变性，促进有益肠道微生物的生长，并维持 HFD 小鼠的脂代谢。此外，AAM 还可激活 AMP 激活的蛋白激酶（AMPK）信号通路，体外和体内调节脂肪生成转录因子及其靶基因。综上所述，本研究数据表明，AAM 具有抗脂肪生成活性，部分是通过 AMPK 通路和肠道微生物群调节来实现的。本研究为 AAM 调节脂代谢提供了新的见解，并表明 AAM 对高血脂症具有潜在的治疗作用。
