Deng Xianfeng, Chen Bi, Luo Qin, Zao Xingru, Liu Haizhe, Li Yongqiang
College of Food Science and Technology, Yunnan Agricultural University, Kunming, China.
School of Life and Health Science, Kaili University, Kaili, China.
Front Nutr. 2022 Aug 4;9:933068. doi: 10.3389/fnut.2022.933068. eCollection 2022.
Obesity is characterized by excessive lipid accumulation, hypertrophy, and hyperplasia of adipose cells. Hulless barley ( L. var. nudum Hook. f.) is the principal crop grown in the Qinghai-Tibet plateau. Polyphenols, the major bioactive compound in hulless barley, possess antioxidant, anti-inflammatory, and antibacterial properties. However, the anti-obesity effect of hulless barley polyphenol (HBP) extract has not been explored. Therefore, the current study assessed the impact of HBP extract on preventing obesity. For this purpose, we evaluated the inhibitory effect of HBP extract against obesity-related enzymes. Moreover, we investigated the effect of HBP extract on adipocyte differentiation and adipogenesis through 3T3-L1 adipocytes. Our results demonstrated that HBP extract could inhibit α-amylase, α-glucosidase (α-GLU), and lipase in a dose-dependent manner. In addition, HBP extract inhibited the differentiation of 3T3-L1 preadipocytes by arresting the cell cycle at the G0/G1 phase. Furthermore, the extract suppressed the expression of adipogenic transcription factors such as peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα), regulating fatty acid synthase (FAS), fatty acid-binding protein 4 (FABP4), and adipose triglyceride lipase (ATGL). It was also observed that HBP extract alleviated intracellular lipid accumulation by attenuating oxidative stress. These findings specify that HBP extract could inhibit obesity-related enzymes, adipocyte differentiation, and adipogenesis. Therefore, it is potentially beneficial in preventing obesity.
肥胖的特征是脂质过度积累、脂肪细胞肥大和增生。青稞（L. var. nudum Hook. f.）是青藏高原种植的主要作物。多酚是青稞中的主要生物活性化合物，具有抗氧化、抗炎和抗菌特性。然而，青稞多酚（HBP）提取物的抗肥胖作用尚未得到探索。因此，本研究评估了HBP提取物对预防肥胖的影响。为此，我们评估了HBP提取物对肥胖相关酶的抑制作用。此外，我们通过3T3-L1脂肪细胞研究了HBP提取物对脂肪细胞分化和脂肪生成的影响。我们的结果表明，HBP提取物可以剂量依赖性方式抑制α-淀粉酶、α-葡萄糖苷酶（α-GLU）和脂肪酶。此外，HBP提取物通过将细胞周期阻滞在G0/G1期来抑制3T3-L1前脂肪细胞的分化。此外，该提取物抑制了过氧化物酶体增殖物激活受体γ（PPARγ）、CCAAT/增强子结合蛋白α（C/EBPα）、调节脂肪酸合酶（FAS）、脂肪酸结合蛋白4（FABP4）和脂肪甘油三酯脂肪酶（ATGL）等脂肪生成转录因子的表达。还观察到，HBP提取物通过减轻氧化应激来减轻细胞内脂质积累。这些发现表明，HBP提取物可以抑制肥胖相关酶、脂肪细胞分化和脂肪生成。因此，它在预防肥胖方面可能具有益处。
