Servicio de Bioquímica-Investigación, Hospital Universitario Ramón y Cajal - IRYCIS, Madrid, Spain.
CIBER de Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.
Methods Mol Biol. 2021;2344:119-135. doi: 10.1007/978-1-0716-1562-1_9.
Peptide microarrays have been used to study protein-protein interaction, enzyme-substrate profiling, epitope mapping, vaccine development, and immuno-profiling. Unlike proteins, peptides are cheap to produce, and can be produced in a high-throughput manner, in a reliable and consistent procedure that reduces batch-to-batch variability. All this provides the peptide microarrays a great potential in the development of new diagnostic tools. Noncontact printing, such as piezoelectric systems, results in a considerable advance in protein and peptide microarray production. In particular, they improve drop deposition, sample distribution, quality control, and flexibility in substrate deposition and eliminate cross-contamination and carryover. These features contribute to creating reproducible assays and generating more reliable data. Here we describe the methods and materials for epitope mapping of food allergens using peptide microarrays produced with a noncontact piezoelectric microarray printer.
肽微阵列已被用于研究蛋白质-蛋白质相互作用、酶-底物分析、表位作图、疫苗开发和免疫分析。与蛋白质不同，肽的生产成本低廉，并且可以以高通量的方式生产，在可靠且一致的过程中，减少批间变异性。所有这些都为开发新的诊断工具提供了巨大的潜力。非接触式打印，如压电系统，在蛋白质和肽微阵列的生产中取得了相当大的进展。特别是，它们改善了液滴沉积、样品分布、质量控制和底物沉积的灵活性，并消除了交叉污染和携带污染。这些特性有助于创建可重复的检测，并生成更可靠的数据。在这里，我们描述了使用非接触式压电微阵列打印机生产的肽微阵列进行食物过敏原表位作图的方法和材料。
