Immunology Department, Centre de Diagnòstic Biomèdic, Hospital Clínic de Barcelona, Barcelona, Spain.
Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, Barcelona, Spain.
Methods Mol Biol. 2021;2344:107-117. doi: 10.1007/978-1-0716-1562-1_8.
Food allergy is becoming a great problem in industrialized countries. Thus, there is the need for a robust understanding of all aspects characterizing IgE response to allergens. The epitope mapping of B-cell epitopes has the potential to become a fundamental tool for food allergy diagnosis and prognosis and to lead to a better understanding of the pathogenesis. Using this approach, we have worked on epitope mapping of the most important plant food allergens identified in the Mediterranean area. The final aim of this study is to define the immune response regarding B epitopes and its clinical relevance in LTP allergy. This chapter describes the protocol to produce microarrays using a library of overlapping peptides corresponding to the primary sequences of allergenic lipid transfer proteins.
食物过敏在工业化国家正成为一个大问题。因此，需要深入了解所有与 IgE 对过敏原反应有关的方面。B 细胞表位的表位作图有可能成为食物过敏诊断和预后的基本工具，并有助于更好地了解发病机制。我们采用这种方法，对在地中海地区发现的最重要的植物食物过敏原进行了 B 细胞表位作图。本研究的最终目标是确定 LTP 过敏中 B 表位的免疫反应及其临床相关性。本章描述了使用对应于过敏脂质转移蛋白的一级序列的重叠肽库来制作微阵列的方案。
