Xiao Yong, An Xing-Kui, Khashaveh Adel, Shan Shuang, Wang Qi, Wang Shan-Ning, Gu Shao-Hua, Li Zhen-Yu, Zhang Yong-Jun
Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
J Agric Food Chem. 2020 Nov 25;68(47):13815-13823. doi: 10.1021/acs.jafc.0c04434. Epub 2020 Nov 5.
Plant volatiles such as floral scent compounds play a crucial role in mediating insect host locating, mate search, and oviposition sites selection. The alfalfa plant bug, (Goeze), is a seriously polyphagous herbivore of alfalfa and cotton that has an obvious preference for flowering host plants. In this study, we focused on the role of an odorant receptor AlinOR59 in the perception of plant volatiles in . hybridization showed that was coexpressed with the coreceptor in the ORNs cell located in the long curved sensilla trichodea on antennae of both genders. The oocytes expression coupled with two-electrode voltage clamp recordings demonstrated that AlinOR59 responded to 15 plant volatiles. In electroantennogram assays, all of the above 15 compounds could excite electrophysiological responses in the antennae of adult bugs. Furthermore, an important floral scent compound, methyl salicylate, was utilized to evaluate the behavioral responses of . It was found that adult bugs of both genders were significantly attracted to methyl salicylate. Taken together, our findings suggest that AlinOR59 plays a crucial role in the perception of floral scents in and could be used as a potential target to design novel olfactory regulators for the management of bugs.
植物挥发物，如花香化合物，在介导昆虫寻找寄主、寻找配偶和选择产卵地点方面起着至关重要的作用。苜蓿盲蝽（Goeze）是苜蓿和棉花的一种严重多食性食草动物，对开花寄主植物有明显偏好。在本研究中，我们聚焦于气味受体AlinOR59在苜蓿盲蝽感知植物挥发物中的作用。杂交实验表明，AlinOR59与共受体在雌雄两性触角上长弯形毛形感器中的嗅觉感受神经元（ORN）细胞中共同表达。卵母细胞表达结合双电极电压钳记录表明，AlinOR59对15种植物挥发物有反应。在触角电位测定中，上述所有15种化合物均可激发成年盲蝽触角的电生理反应。此外，一种重要的花香化合物水杨酸甲酯被用于评估苜蓿盲蝽的行为反应。结果发现，雌雄成年盲蝽均被水杨酸甲酯显著吸引。综上所述，我们的研究结果表明，AlinOR59在苜蓿盲蝽感知花香中起关键作用，可作为设计新型嗅觉调节剂以防治盲蝽的潜在靶点。
