Woolley J L, Lau B H, Ruckle H C, Torrey R R
Department of Urology, School of Medicine, Loma Linda University, CA 92350.
J Urol. 1988 Sep;140(3):660-3. doi: 10.1016/s0022-5347(17)41750-2.
Postsurgical immunochemotherapy with Corynebacterium parvum (CP) and cis-diamminedichloroplatinum (II) (CDDP) was evaluated in mice with transitional cell carcinoma (MBT-2). C3H/He mice were transplanted subcutaneously in the hind limb with 5 x 10(5) tumor cells. Ten to 14 days later when the tumor reached a diameter of five to seven mm., it was surgically removed. Mice were then randomized into four groups to receive a total of three treatments on days 1, 3 and 5 after surgery: 1) saline (control group); 2) CP, 250 micrograms. into the surgical site; 3) CDDP, 5 micrograms./gm. body weight intraperitoneally; and 4) combined CP and CDDP. Recurrence of tumor occurred in 70%, 52%, 55% and 28% of mice receiving surgery only, CP, CDDP, and combined CP and CDDP respectively. In the second part of the experiment, phagocytic activity using chemiluminescence assay and natural killer (NK) activity using chromium-51 release assay were determined with cells from the peritoneum, spleen and inguinal lymph nodes. CP or CDDP alone enhanced the phagocytic and NK activity. The most significant enhancement was obtained with cells from the inguinal lymph nodes of mice receiving combined CP and CDDP, the group with the lowest tumor recurrence. These results suggest that combination of CP and CDDP may be useful in control of postsurgical recurrence of bladder cancer.
对患有移行细胞癌（MBT-2）的小鼠进行了短小棒状杆菌（CP）和顺二氨二氯铂（II）（CDDP）的术后免疫化疗评估。将5×10⁵个肿瘤细胞皮下移植到C3H/He小鼠的后肢。10至14天后，当肿瘤直径达到5至7毫米时，将其手术切除。然后将小鼠随机分为四组，在手术后第1、3和5天共接受三种治疗：1）生理盐水（对照组）；2）CP，250微克，注入手术部位；3）CDDP，5微克/克体重，腹腔内注射；4）CP和CDDP联合使用。仅接受手术、CP、CDDP以及CP和CDDP联合治疗的小鼠中，肿瘤复发率分别为70%、52%、55%和28%。在实验的第二部分，使用化学发光测定法测定吞噬活性，并使用铬-51释放测定法测定来自腹膜、脾脏和腹股沟淋巴结的细胞的自然杀伤（NK）活性。单独使用CP或CDDP可增强吞噬和NK活性。在接受CP和CDDP联合治疗的小鼠的腹股沟淋巴结细胞中获得了最显著的增强，该组肿瘤复发率最低。这些结果表明，CP和CDDP联合使用可能有助于控制膀胱癌术后复发。
