Lau B H, Woolley J L, Marsh C L, Barker G R, Koobs D H, Torrey R R
J Urol. 1986 Sep;136(3):701-5. doi: 10.1016/s0022-5347(17)45031-2.
Immunotherapy with bacillus Calmette-Guerin (BCG), Corynebacterium parvum (CP), keyhole limpet hemocyanin (KLH) and an extract of Allium sativum (AS) was studied in a transitional cell carcinoma (MBT-2) in mice. Comparison was made between intraperitoneal (IP) versus intralesional (IL) administration of these agents. C3H/He mice were transplanted subcutaneously in the hind limb with 5 X 10(4) tumor cells. After transplantation, mice were randomized into groups to receive either IP or IL treatments with BCG (2 X 10(6) CFU), CP (250 micrograms.), KLH (50 micrograms.) or AS (25 mg.). At weekly intervals the tumor volume was determined. To assess the local cellular events following these treatments, histopathological studies were performed 10 days after tumor transplant with tissues removed from the injected sites. IL route was much more effective than IP route in inhibiting tumor growth. CP and AS exhibited more significant therapeutic effect than BCG or KLH. No tumor developed in mice which received five IL treatments of CP or AS. The data indicate that CP or AS may serve as effective biological response modifiers in controlling transitional cell carcinoma. The study further emphasizes that route and frequency of administration are crucial variables determining efficacy of immunotherapy.
在小鼠移行细胞癌（MBT - 2）中研究了用卡介苗（BCG）、短小棒状杆菌（CP）、钥孔戚血蓝蛋白（KLH）和大蒜提取物（AS）进行免疫治疗。对这些药物的腹腔内（IP）给药与瘤内（IL）给药进行了比较。将5×10⁴个肿瘤细胞皮下移植到C3H/He小鼠的后肢。移植后，将小鼠随机分组，接受BCG（2×10⁶CFU）、CP（250微克）、KLH（50微克）或AS（25毫克）的IP或IL治疗。每周测定肿瘤体积。为了评估这些治疗后的局部细胞事件，在肿瘤移植10天后，对从注射部位取出的组织进行组织病理学研究。在抑制肿瘤生长方面，IL途径比IP途径更有效。CP和AS比BCG或KLH表现出更显著的治疗效果。接受5次CP或AS的IL治疗的小鼠未发生肿瘤。数据表明，CP或AS可作为控制移行细胞癌的有效生物反应调节剂。该研究进一步强调，给药途径和频率是决定免疫治疗疗效的关键变量。
