Performance of nanoScan PET/CT and PET/MR for quantitative imaging of F and Zr as compared with ex vivo biodistribution in tumor-bearing mice.

INTRODUCTION

The assessment of ex vivo biodistribution is the preferred method for quantification of radiotracers biodistribution in preclinical models, but is not in line with current ethics on animal research. PET imaging allows for noninvasive longitudinal evaluation of tracer distribution in the same animals, but systemic comparison with ex vivo biodistribution is lacking. Our aim was to evaluate the potential of preclinical PET imaging for accurate tracer quantification, especially in tumor models.

METHODS

NEMA NU 4-2008 phantoms were filled with C, Ga, F, or Zr solutions and scanned in Mediso nanoPET/CT and PET/MR scanners until decay. N87 tumor-bearing mice were i.v. injected with either [F]FDG (~ 14 MBq), kept 50 min under anesthesia followed by imaging for 20 min, or with [Zr]Zr-DFO-NCS-trastuzumab (~ 5 MBq) and imaged 3 days post-injection for 45 min. After PET acquisition, animals were killed and organs of interest were collected and measured in a γ-counter to determine tracer uptake levels. PET data were reconstructed using TeraTomo reconstruction algorithm with attenuation and scatter correction and regions of interest were drawn using Vivoquant software. PET imaging and ex vivo biodistribution were compared using Bland-Altman plots.

RESULTS

In phantoms, the highest recovery coefficient, thus the smallest partial volume effect, was obtained with F for both PET/CT and PET/MR. Recovery was slightly lower for C and Zr, while the lowest recovery was obtained with Ga in both scanners. In vivo, tumor uptake of the F- or Zr-labeled tracer proved to be similar irrespective whether quantified by either PET/CT and PET/MR or ex vivo biodistribution with average PET/ex vivo ratios of 0.8-0.9 and a deviation of 10% or less. Both methods appeared less congruent in the quantification of tracer uptake in healthy organs such as brain, kidney, and liver, and depended on the organ evaluated and the radionuclide used.

CONCLUSIONS

Our study suggests that PET quantification of F- and Zr-labeled tracers is reliable for the evaluation of tumor uptake in preclinical models and a valuable alternative technique for ex vivo biodistribution. However, PET and ex vivo quantification require fully described experimental and analytical procedures for reliability and reproducibility.