Ribeiro F M, Correia P M M, Santos A C, Veloso J F C A
Institute for Nanostructures, Nanomodelling and Nanofabrication (i3N), Department of Physics, University of Aveiro (DFis-UA), 3810-193, Aveiro, Portugal.
Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine of the University of Coimbra (FMUC), Area of Environment Genetics and Oncobiology (CIMAGO), Center for Innovative Biomedicine and Biotechnology (CIBB), 3000-548, Coimbra, Portugal.
EJNMMI Res. 2022 Aug 13;12(1):49. doi: 10.1186/s13550-022-00921-y.
The experimental outcomes of small-animal positron emission tomography (PET) imaging with F-labelled fluorodeoxyglucose (F-FDG) can be particularly compromised by animal preparation and care. Several works intend to improve research reporting and amplify the quality and reliability of published research. Though these works provide valuable information to plan and conduct animal studies, manuscripts describe different methodologies-standardization does not exist. Consequently, the variation in details reported can explain the difference in the experimental results found in the literature. Additionally, the resources and guidelines defining protocols for small-animal imaging are scarce, making it difficult for researchers to obtain and compare accurate and reproducible data. Considering the selection of suitable procedures key to ensure animal welfare and research improvement, this paper aims to prepare the way for a future guideline on mice preparation and care for PET imaging with F-FDG. For this purpose, a global standard protocol was created based on recommendations and good practices described in relevant literature.
用F标记的氟脱氧葡萄糖（F-FDG）进行小动物正电子发射断层扫描（PET）成像的实验结果可能会因动物的准备和护理工作而受到特别影响。有几项研究旨在改进研究报告，并提高已发表研究的质量和可靠性。尽管这些研究为规划和开展动物研究提供了有价值的信息，但手稿描述的方法各不相同——不存在标准化。因此，所报告细节的差异可以解释文献中发现的实验结果的差异。此外，定义小动物成像方案的资源和指南稀缺，这使得研究人员难以获取和比较准确且可重复的数据。考虑到选择合适的程序是确保动物福利和研究改进的关键，本文旨在为未来关于用F-FDG进行PET成像的小鼠准备和护理指南铺平道路。为此，基于相关文献中描述的建议和良好做法创建了一个全球标准方案。
