School of Chemistry and Chemical Engineering, Anhui University, Hefei, China.
Methods Mol Biol. 2021;2275:265-277. doi: 10.1007/978-1-0716-1262-0_16.
Multifunctional nanoplatforms are promising scaffolds for biomedical applications such as bioimaging, chemical/biological sensors, drug delivery, and cancer diagnosis and/or treatments. Mitochondria play crucial roles in metabolism of eukaryotic cells; therefore, mitochondria-targeting molecule such as triphenylphosphonium (TPP) is attached onto the magnetic mesoporous silica nanoparticle (FeO@mSiO). In order to track the nanoparticles, fluorescent carbon quantum dots (CDs) were conjugated to the FeO@mSiO. The as-constructed FeO@mSiO-TPP/CQD nanoplatform showed minimal cytotoxicity in various cell lines such as A549, CHO, HeLa, SH-SY5Y, HFF, and HMEC-1. External magnetic field-assisted uptake of the nanoplatform by tumor cell has been achieved promptly. More importantly, conjugation with CQDs endows the nanoplatform multicolored fluorescence that can remain bright and stable inside cells for a long time. This nanoplatform provides a multifunctional platform in targeting, imaging, and agent delivery for mitochondria-related disease diagnosis and treatment.
多功能纳米平台在生物医学应用中具有广阔的前景，如生物成像、化学/生物传感器、药物输送以及癌症的诊断和/或治疗。线粒体在真核细胞的代谢中起着至关重要的作用；因此，将靶向线粒体的分子（如三苯基膦（TPP））连接到磁性介孔硅纳米颗粒（FeO@mSiO）上。为了跟踪纳米颗粒，将荧光碳量子点（CDs）连接到 FeO@mSiO 上。构建的 FeO@mSiO-TPP/CQD 纳米平台在各种细胞系（如 A549、CHO、HeLa、SH-SY5Y、HFF 和 HMEC-1）中表现出最小的细胞毒性。通过外部磁场辅助，肿瘤细胞可以迅速摄取纳米平台。更重要的是，与 CDs 的结合赋予了纳米平台多色荧光，这种荧光可以在细胞内长时间保持明亮和稳定。该纳米平台为与线粒体相关的疾病的诊断和治疗提供了一种靶向、成像和递药的多功能平台。
