Su Xi, Xu Yi, Che Yulan, Liao Xin, Jiang Yan
Department of Pharmaceutical Chemistry, Chemistry and Chemical Engineering College, Chongqing University, Chongqing, 400044, China.
Department of Biological Chemistry, Defense Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, Chongqing, 400044, China.
J Biomed Mater Res A. 2015 Dec;103(12):3956-64. doi: 10.1002/jbm.a.35468. Epub 2015 Sep 26.
A new type of multifunctional fluorescent magnetic carbon quantum dots SPIO@CQDs(n) ([superparamagnetic iron oxide nanoparticles (SPIO), carbon quantum dots, (CQDs)]) with magnetic and fluorescence properties was designed and prepared through layer-by-layer self-assembly method. The as-synthesized SPIO@CQDs(n) exhibited different emission colors including blue, green, and red when they were excited at different excitation wavelengths, and its fluorescent intensity increased as the increase of CQD layer (n). SPIO@CQDs(n) with quite low toxicity could mark cytoplasm with fluorescence by means of nonimmune markers. The mixture sample of liver cells L02 and hepatoma carcinoma cells HepG2 was taken as an example, and HepG2 cells were successfully separated and detected effectively by SPIO@CQDs(n), with a separation rate of 90.31%. Importantly, the designed and prepared SPIO@CQDs( n ) are certified to be wonderful biological imaging and magnetic separation regents.
通过层层自组装法设计并制备了一种具有磁性和荧光性质的新型多功能荧光磁性碳量子点SPIO@CQDs(n)（[超顺磁性氧化铁纳米颗粒（SPIO），碳量子点（CQDs）]）。合成的SPIO@CQDs(n)在不同激发波长下激发时呈现出包括蓝色、绿色和红色在内的不同发射颜色，并且其荧光强度随着CQD层数（n）的增加而增强。具有相当低毒性的SPIO@CQDs(n)可以通过非免疫标记以荧光标记细胞质。以肝细胞L02和肝癌细胞HepG2的混合样本为例，SPIO@CQDs(n)成功地分离并有效检测了HepG2细胞，分离率为90.31%。重要的是，所设计和制备的SPIO@CQDs(n)被证明是出色的生物成像和磁性分离试剂。
