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有机和传统种植农产品中弓形虫卵囊的检测。

Detection of Toxoplasma gondii oocysts on organic and conventionally grown produce.

机构信息

Virginia Military Institute, Biology Department, Lexington, VA, USA.

Virginia Military Institute, Biology Department, Lexington, VA, USA.

出版信息

Food Microbiol. 2021 Oct;99:103798. doi: 10.1016/j.fm.2021.103798. Epub 2021 May 13.

Abstract

Toxoplasma gondii infection can result in toxoplasmosis and potential psychological effects. Research commonly focuses on infection through contact with cat fecal matter or consumption of contaminated meat. However, T. gondii oocysts can persist in the environment for years and may be present in soils and on soil-grown produce. Rates of oocyst DNA recovery from produce were high, with 18% of vegetable samples testing positive for T. gondii via PCR test and melt curve analysis. Radishes had significantly higher oocyst counts than arugula, collard greens, kale, lettuce, and spinach. There were no significant differences in oocyst detection rates between samples taken from organic farmer's markets and conventional grocery stores. This study demonstrates that these oocysts can transfer to produce grown both conventionally and using organic techniques.

摘要

刚地弓形虫感染可导致弓形体病和潜在的心理影响。研究通常集中在通过接触猫粪便或食用受污染的肉类而感染。然而,刚地弓形虫卵囊可以在环境中持续存在多年,并且可能存在于土壤中和土壤种植的农产品上。从农产品中回收卵囊 DNA 的比率很高,18%的蔬菜样本通过 PCR 检测和熔解曲线分析呈刚地弓形虫阳性。萝卜的卵囊数明显高于芝麻菜、羽衣甘蓝、羽衣甘蓝、生菜和菠菜。从有机农贸市场和传统杂货店采集的样本中,卵囊检测率没有显著差异。本研究表明,这些卵囊可以转移到常规和有机技术种植的农产品上。

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