Moreno-Mesonero L, Soler L, Amorós I, Moreno Y, Ferrús M A, Alonso J L
Instituto de Ingeniería del Agua y Medio Ambiente (IIAMA), Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain.
Departamento de Biotecnología, Centro Avanzado de Microbiología de Alimentos, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain.
Food Waterborne Parasitol. 2023 Jun 22;32:e00200. doi: 10.1016/j.fawpar.2023.e00200. eCollection 2023 Sep.
In this study, the presence of spp., sp., spp., sp., and was assessed in organic leafy green vegetables (lettuce, spinach, cabbage) and fruits (strawberry), which are usually consumed raw. A total of 110 organic samples were collected in Valencia (Spain). Protozoa were concentrated before detection by immunofluorescence ( spp. and sp.) or real-time qPCR ( spp., sp., , , and ). The most abundant protozoa in organic vegetables and berry fruits were (65.5%), followed by (37.2%), (17.3%), (12.7%), spp. (6.8%), sp. (1.8%) and sp. (1.7%). was not found in any of the organic samples. Thus, results showed that consumers can be exposed to protozoan parasites by consuming organic vegetables and berry fruits. This is the first report in Spain describing the presence of the protozoan pathogens spp., sp., , and , spp. and sp. in organic fresh produce. The results of this research will help determine the risk of foodborne protozoan parasites on organic leafy greens and strawberries that are available at local markets.
在本研究中，对通常生食的有机绿叶蔬菜（生菜、菠菜、卷心菜）和水果（草莓）中 spp.、 sp.、 spp.、 sp. 以及 的存在情况进行了评估。在西班牙巴伦西亚共采集了110份有机样本。原生动物在检测前通过免疫荧光法（ spp. 和 sp.）或实时定量PCR法（ spp.、 sp.、 、 、 和 ）进行浓缩。有机蔬菜和浆果类水果中最常见的原生动物是 （65.5%），其次是 （37.2%）、 （17.3%）、 （12.7%）、 spp.（6.8%）、 sp.（1.8%）和 sp.（1.7%）。在任何有机样本中均未发现 。因此，结果表明消费者通过食用有机蔬菜和浆果类水果可能接触到原生动物寄生虫。这是西班牙首份描述有机新鲜农产品中存在原生动物病原体 spp.、 sp.、 、 和 、 spp. 以及 sp. 的报告。本研究结果将有助于确定当地市场上有机绿叶蔬菜和草莓中食源性原生动物寄生虫的风险。
