University of Naples Federico II, Naples, Italy.
An-Najah National University, Nablus, Palestine.
PeerJ. 2024 Mar 26;12:e16973. doi: 10.7717/peerj.16973. eCollection 2024.
The discovery of plant-derived compounds that are able to combat antibiotic-resistant pathogens is an urgent demand. Over years, attracted considerable attention because of its beneficial medical properties. Phytochemical analyses revealed that plant species contain several metabolites, such as sesquiterpene lactones (STLs), essential oils, flavonoids, alkaloids, and lignans.The organic extract of plant, collected in Palestine, showed significant antimicrobial properties towards a panel of Gram-negative and Gram-positive bacterial strains when the Minimal Inhibitory Concentration (MIC) values were evaluated by broth microdilution assays. A bio-guided fractionation of the active extract multiple steps of column and thin layer chromatography allowed us to obtain three main compounds. The isolated metabolites were identified as the STLs cnicin, 11β,13-dihydrosalonitenolide and salonitenolide by spectroscopic and spectrometric analyses. Cnicin conferred the strongest antimicrobial activity among the identified compounds. Moreover, the evaluation of its antibiofilm activity by biomass assays through crystal violet staining revealed almost 30% inhibition of biofilm formation in the case of ATCC 17878 strain. Furthermore, the quantification of carbohydrates and proteins present in the extracellular polymeric substance (EPS) revealed the ability of cnicin to significantly perturb biofilm structure. Based on these promising results, further investigations might open interesting perspectives to its applicability in biomedical field to counteract multidrug resistant infections.
发现能够对抗抗生素耐药病原体的植物衍生化合物是当务之急。多年来， 因其有益的医学特性而引起了相当大的关注。植物化学分析表明， 植物物种含有几种代谢物，如倍半萜内酯 (STLs)、精油、类黄酮、生物碱和木脂素。从巴勒斯坦采集的 植物的有机提取物在肉汤微量稀释测定中评估最小抑菌浓度 (MIC) 值时，对一组革兰氏阴性和革兰氏阳性细菌菌株表现出显著的抗菌特性。活性提取物的生物导向分步分离 通过柱层析和薄层色谱的多步分离，我们获得了三种主要化合物。通过光谱和光谱分析鉴定分离出的代谢物为 STLs 千里光宁、11β,13-二氢萨罗替宁内酯和萨罗替宁内酯。千里光宁在鉴定出的化合物中表现出最强的抗菌活性。此外，通过结晶紫染色生物量测定评估其抗生物膜活性，发现 ATCC 17878 菌株的生物膜形成抑制率接近 30%。此外，对细胞外聚合物 (EPS) 中存在的碳水化合物和蛋白质进行定量分析表明，千里光宁能够显著破坏生物膜结构。基于这些有希望的结果，进一步的研究可能会为其在生物医学领域应用于对抗多药耐药感染开辟有趣的前景。
