Anopheles (Nyssorhynchus) tadei: A new species of the Oswaldoi-konderi complex (Diptera, Anophelinae) and its morphological and molecular distinctions from An. konderi sensu stricto.

The Oswaldoi-konderi Complex (Anopheles, Nyssorhynchus) is composed of five species that have been distinguished and delimited using DNA sequences of mitochondrial and nuclear genes. At least two species of the complex have been formally described, namely Anopheles oswaldoi s.s. and An. konderi; however, the identity of An. konderi s.s. is unclear because two morphologically similar species co-exist in the type-locality in the municipality of Coari, Amazonas state, Brazil. Moreover, the study of resurrection and designation of the neotype of An. konderi s.s. included a mixture of both forms. In the present study, mosquitoes were collected in Coari to establish the molecular identity of An. konderi s.s. and describe a new species based on morphological and molecular data. Six females were collected and separated individually for oviposition. The parental progenies were obtained from field collected females, fourth-instar larva, pupa, and female and male were employed for morphological characterization. Genomic DNA from one fourth-instar larva of each progeny was extracted and sequenced for the mtDNA COI barcode region, CAD gene, and the ITS2 rDNA nuclear region to establish the molecular identity of the two morphological forms of An. konderi s.l. The An. konderi neotype was re-examined. The morphological and molecular analyses revealed two distinct groups: the first group was identical to the neotype of An. konderi s.s., whereas the second was found to belong to the group informally referred to as An. sp. near konderi or An. konderi B, herein described as Anopheles tadei n. sp.