高通量表面标志物筛选原发性人乳腺组织揭示了更多的细胞异质性。

High-throughput surface marker screen on primary human breast tissues reveals further cellular heterogeneity.

机构信息

CRUK Cambridge Institute, University of Cambridge, Cambridge, CB2 0RE, UK.

Cambridge Institute for Medical Research, Cambridge University, Cambridge, CB2 0XY, UK.

出版信息

Breast Cancer Res. 2021 Jun 13;23(1):66. doi: 10.1186/s13058-021-01444-5.

DOI:10.1186/s13058-021-01444-5

PMID:34120626

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8201685/

Abstract

BACKGROUND

Normal human breast tissues are a heterogeneous mix of epithelial and stromal subtypes in different cell states. Delineating the spectrum of cellular heterogeneity will provide new insights into normal cellular properties within the breast tissue that might become dysregulated in the initial stages of cancer. Investigation of surface marker expression provides a valuable approach to resolve complex cell populations. However, the majority of cell surface maker expression of primary breast cells have not been investigated.

METHODS

To determine the differences in expression of a range of uninvestigated cell surface markers between the normal breast cell subpopulations, primary human breast cells were analysed using high-throughput flow cytometry for the expression of 242 cell surface proteins in conjunction with EpCAM/CD49f staining.

RESULTS

We identified 35 surface marker proteins expressed on normal breast epithelial and/or stromal subpopulations that were previously unreported. We also show multiple markers were equally expressed in all cell populations (e.g. CD9, CD59, CD164) while other surface markers were confirmed to be enriched in different cell lineages: CD24, CD227 and CD340 in the luminal compartment, CD10 and CD90 in the basal population, and CD34 and CD140b on stromal cells.

CONCLUSIONS

Our dataset of CD marker expression in the normal breast provides better definition for breast cellular heterogeneity.

摘要

背景

正常人体乳腺组织是不同细胞状态的上皮和基质亚型的异质混合物。描绘细胞异质性的范围将为乳腺组织中正常细胞特性提供新的见解，这些特性可能在癌症的早期阶段失调。表面标记物表达的研究为解析复杂的细胞群体提供了一种有价值的方法。然而，大多数原发性乳腺细胞的细胞表面标记物的表达尚未被研究过。

方法

为了确定一系列未被研究的细胞表面标记物在正常乳腺细胞亚群中的表达差异，我们使用高通量流式细胞术分析了原发性人乳腺细胞，检测了 242 种细胞表面蛋白的表达情况，并结合 EpCAM/CD49f 染色。

结果

我们鉴定了 35 种在正常乳腺上皮和/或基质亚群中表达的表面标记蛋白，这些蛋白以前没有被报道过。我们还发现，多个标记物在所有细胞群体中均有相同表达（例如 CD9、CD59、CD164），而其他表面标记物被证实富集在不同的细胞谱系中：CD24、CD227 和 CD340 在腔室中，CD10 和 CD90 在基底群体中，CD34 和 CD140b 在基质细胞中。