Department of Natural Resources, Faculty of African Post Graduate Studies, Cairo University, Cairo, 12613, Egypt.
Biochemistry Department, King Khalid University, Abha, 61321, Saudi Arabia.
Environ Sci Pollut Res Int. 2021 Oct;28(39):55089-55101. doi: 10.1007/s11356-021-14856-9. Epub 2021 Jun 14.
On juveniles of Oreochromis niloticus, the protective potential of ascorbic acid (Asc) against oxidative stress and genotoxicity induced by acetamiprid (Aceta) sub-lethal concentrations was investigated in this study. Fishes were divided into six groups and exposed to either Asc (50 ppm), 10 and 20 ppm Aceta, 10 ppm (Aceta)+Asc, 20 ppm (Aceta)+Asc, or the unexposed control group. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) activities and their transcripts were assessed. DNA damage in erythrocytes, hepatocytes, and gill cells, in addition to the mitotic index (MI), and the existence of erythrocytic nuclear abnormalities (ENAs) were performed. The results showed that concentrations of Aceta (10 and 20 ppm) induced oxidative stress by altering the antioxidant enzyme activities and transcripts. There were genotoxic effects of Aceta exposure showed by the significant (P < 0.05) increase in DNA-damaged cells and ENA, meanwhile a decrease in MI. Co-exposure with Asc showed significant alleviations of oxidative status and genotoxicity. Thus, results suggest that Asc-combined exposure could be the effective treatment against Aceta-induced oxidative stress accompanied with genotoxicity in O. niloticus.
本研究旨在探讨抗坏血酸（Asc）对亚致死浓度乙酰甲胺磷（Aceta）诱导的尼罗罗非鱼幼鱼氧化应激和遗传毒性的保护作用。将鱼类分为六组，分别暴露于 Asc（50ppm）、10ppm 和 20ppm Aceta、10ppm（Aceta）+Asc、20ppm（Aceta）+Asc 或未暴露于对照组。评估超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）的活性及其转录本。测定红细胞、肝细胞和鳃细胞的 DNA 损伤，以及有丝分裂指数（MI）和红细胞核异常（ENAs）的存在。结果表明，Aceta（10 和 20ppm）浓度通过改变抗氧化酶活性和转录本而诱导氧化应激。Aceta 暴露具有遗传毒性作用，表现为 DNA 损伤细胞和 ENA 的显著增加（P<0.05），同时 MI 降低。与 Asc 共同暴露显示出氧化状态和遗传毒性的显著缓解。因此，结果表明 Asc 联合暴露可能是治疗尼罗罗非鱼氧化应激伴随遗传毒性的有效方法。
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