Ghasemi Fereshteh, Khoshmirsafa Majid, Safari Elahe, Asgari Marzieh, Alemrajabi Mehdi, Nojehdehi Shahrzad, Khorrami Samane
Department of Medical Immunology, School of Medicine, Iran University of Medical Science, Tehran, Iran.
Department of General Surgery, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Stem Cell Investig. 2021 May 7;8:9. doi: 10.21037/sci-2020-008. eCollection 2021.
Mesenchymal stem cells (MSCs) with immunoregulatory properties affect immune systems. Many studies showed that antioxidants such as vitamin E (Vit E) and selenium (Se) could improve stem cells survival. This study aims to investigate the effects of MSC conditioned media (CM) treated with Vit E and Se on immune cells.
MSCs were isolated and cultured with Vit E and Se. Immature dendritic cells (DCs) and peripheral blood mononuclear cells (PBMCs) were cultured with MSC CM treated with Vit E and Se. The expression of HLA-DR, CD86, CD40, and CD83 on mature DC were evaluated. DC supernatant and PBMCs supernatant was collected for the study of TGF-β, IL-10, and IL-12. PBMCs evaluated for the expression of T-bet, GATA3, RORγt, and FOXP3.
MSC CM increased CD40 on myeloid DC (mDC). CD40 has been decreased in DC treated with MSC (Vit E) and MSC (Se) CM. HLA-DR expression on DCs and IL-12 level were significantly reduced in MSC (Vit E) CM. IL-10 concentration increased in DCs treated with MSC (Vit E) and MSC (Se) CM. Treatment of PBMCs with MSC CM decreased IL-10 level, FOXP3, and RORγt expression. On the other hand, the MSC (Vit E) CM and MSC (Se) CM decreased the IL-10 level and increased IL-12, T-bet, and RORγt.
According to the results, the treatment of MSC with Vit E and Se enhanced the ability of MSCs to inhibit DCs and improved immunomodulatory effects. Concerning the effect of MSC on PBMC, it seems that it increased RORγt expression through monocytes.
具有免疫调节特性的间充质干细胞（MSCs）会影响免疫系统。许多研究表明，抗氧化剂如维生素E（Vit E）和硒（Se）可提高干细胞存活率。本研究旨在探讨经Vit E和Se处理的MSC条件培养基（CM）对免疫细胞的影响。
分离MSCs并用Vit E和Se进行培养。将未成熟树突状细胞（DCs）和外周血单个核细胞（PBMCs）与经Vit E和Se处理的MSC CM一起培养。评估成熟DC上HLA-DR、CD86、CD40和CD83的表达。收集DC上清液和PBMCs上清液用于研究转化生长因子-β（TGF-β）、白细胞介素-10（IL-10）和白细胞介素-12（IL-12）。评估PBMCs中T-bet、GATA3、维甲酸相关孤儿受体γt（RORγt）和叉头框蛋白P3（FOXP3）的表达。
MSC CM增加了髓样DC（mDC）上的CD40。在用MSC（Vit E）和MSC（Se）CM处理的DC中，CD40有所降低。在MSC（Vit E）CM中，DC上的HLA-DR表达和IL-12水平显著降低。在用MSC（Vit E）和MSC（Se）CM处理的DC中，IL-10浓度增加。用MSC CM处理PBMCs可降低IL-10水平、FOXP3和RORγt表达。另一方面，MSC（Vit E）CM和MSC（Se）CM降低了IL-10水平并增加了IL-12、T-bet和RORγt。
根据结果，用Vit E和Se处理MSC增强了MSC抑制DC的能力并改善了免疫调节作用。关于MSC对PBMC的影响，似乎它通过单核细胞增加了RORγt表达。
