检测根际细菌产生铁载体的高通量方法
High-throughput Method for Detecting Siderophore Production by Rhizosphere Bacteria.
作者信息
Gu ShaoHua, Wan Wen, Shao ZhengYing, Zhong Wei
机构信息
Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, National Engineering Research Center for Organic-based Fertilizers, Nanjing Agricultural University, 210095, Nanjing, China.
Center for Quantitative Biology and Peking-Tsinghua Center for Life Sciences, Peking University, 100871, Beijing, China.
出版信息
Bio Protoc. 2021 May 5;11(9):e4001. doi: 10.21769/BioProtoc.4001.
Siderophores, a key substance that microorganisms produce to obtain iron under iron-limited conditions, play an important role in regulating interactions between beneficial bacteria and pathogenic bacteria. A large number of bacteria were isolated from the rhizosphere, and we used the method presented here to assay the siderophore production by these rhizosphere bacteria. This method is a modified version of the universal chrome azurol S (CAS) assay that uses a 96-channel manual pipetting workstation. By combining the liquid CAS assay with the multi-channel pipette workstation, high-throughput and rapid detection of siderophore production can be achieved. In summary, this method can be used to gain a general understanding of siderophore production by rhizosphere bacteria.
铁载体是微生物在铁限制条件下产生以获取铁的关键物质，在调节有益细菌和致病细菌之间的相互作用中发挥重要作用。从根际分离出大量细菌，我们使用此处介绍的方法来测定这些根际细菌产生铁载体的情况。该方法是通用铬天青S（CAS）测定法的改进版本，使用96通道手动移液工作站。通过将液体CAS测定法与多通道移液器工作站相结合，可以实现铁载体产生的高通量和快速检测。总之，该方法可用于全面了解根际细菌产生铁载体的情况。
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