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利用Illumina NGS平台通过鸟枪法测序确定的太平洋帽贝(腹足纲:笠形腹足目)的完整线粒体基因组。

Complete mitochondrial genome of the Pacific limpet (Gastropoda: Patellogastropoda) determined by shotgun sequencing using the Illumina NGS platform.

作者信息

Nakashima Satoko, Shimizu Moe, Hirota Kazuki, Hiruta Shimpei F, Nagisa Nakaji, Fujita Toshihiko, Sasaki Takenori, Setiamarga Davin H E

机构信息

National Institute of Technology (KOSEN), Wakayama College, Wakayama, Japan.

The University Museum, The University of Tokyo, Tokyo, Japan.

出版信息

Mitochondrial DNA B Resour. 2021 Jun 3;6(7):1857-1859. doi: 10.1080/23802359.2021.1934579.

Abstract

The Pacific limpet is one of the most commonly found limpets in the intertidal shores of Japan. Here, we report the full mitogenome sequence of an individual specimen of the species, which was collected from the intertidal rocky beach in the Nada beach of Gobo City, Wakayama, Japan (33.8316 N, 135.1751 E), in 2018. The sequence was determined by the shotgun sequencing method using the NGS Illumina MiSeq platform. The genomic structure of is the same as the previously reported congener, , which shows a representative Nacellidae and metazoan mitogenomic structures. The mitogenome has all of its 37 genes included in its 16,153 bp, with one control region located between the tRNA-Cys and tRNA-Gly genes. In order to clarify the phylogenetic position of in Gastropoda, a data set including the mitogenomes of 10 patellogastropods, 10 non-patellogastropod gastropods, and four outgroups were used in maximum likelihood inferences. Although with some exceptions, the resulting phylogeny supported the monophylies of traditionally accepted gastropod subclasses, and thus confirms the position of in Patellogastropoda.

摘要

太平洋帽贝是日本潮间带海岸最常见的帽贝之一。在此,我们报告了该物种一个个体标本的完整线粒体基因组序列,该标本于2018年采集自日本和歌山县串本町名田海滩的潮间带岩石滩(北纬33.8316,东经135.1751)。该序列通过使用NGS Illumina MiSeq平台的鸟枪法测序方法确定。其基因组结构与先前报道的同属物种相同,显示出典型的笠贝科和后生动物线粒体基因组结构。该线粒体基因组在其16,153 bp中包含所有37个基因,一个控制区位于tRNA-Cys和tRNA-Gly基因之间。为了阐明该物种在腹足纲中的系统发育位置,在最大似然推断中使用了一个数据集,该数据集包括10种笠形腹足类动物、10种非笠形腹足类腹足动物的线粒体基因组以及四个外类群。尽管有一些例外情况,但所得的系统发育树支持了传统上公认的腹足类亚纲的单系性,从而确认了该物种在笠形腹足目中的位置。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3554/8183504/f7d28ea48286/TMDN_A_1934579_F0001_B.jpg

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