Sander K, Lehmann R
Biol. Institüt I (Zoologie), Freiburg, FRG.
Nature. 1988 Sep 1;335(6185):68-70. doi: 10.1038/335068a0.
The segmental pattern of insect embryos depends on influences from morphogenetic centres near each of the egg poles. In Drosophila, maternal effect mutations are known that impair the normal function of each centre. Injection of wild-type cytoplasm into mutant eggs has revealed that morphogenetic signals localized at the anterior and posterior pole of eggs can be transplanted. We show here that these activities can also be detected during oogenesis. Posterior activity can be recovered at an early stage (stage 10, ref. 5) from the oocyte-nurse cell complex, but anterior activity can only be detected in the mature oocytes (stage 14). We conclude that the bicoid-dependent anterior signal, although produced by the nurse cells, does not become active before it is localized to the anterior egg pole, whereas posterior activity can be detected in the nurse cells before, and therefore independently of, its localization to the posterior egg pole.
昆虫胚胎的分节模式取决于来自卵两极附近形态发生中心的影响。在果蝇中，已知母体效应突变会损害每个中心的正常功能。将野生型细胞质注射到突变卵中表明，位于卵前后极的形态发生信号可以移植。我们在此表明，这些活性在卵子发生过程中也能被检测到。后极活性可以在早期阶段（第10阶段，参考文献5）从卵母细胞-滋养细胞复合体中恢复，但前极活性只能在成熟卵母细胞（第14阶段）中检测到。我们得出结论，依赖于bicoid的前极信号虽然由滋养细胞产生，但在定位于卵前极之前不会激活，而后极活性在定位于卵后极之前就能在滋养细胞中检测到，因此与后极定位无关。
