Rothe M, Nauber U, Jäckle H
Institut für Genetik und Mikrobiologie, Universität München, FRG.
EMBO J. 1989 Oct;8(10):3087-94. doi: 10.1002/j.1460-2075.1989.tb08460.x.
The putative finger domain of knirps (kni), a member of the gap class of segmentation genes, was used to isolate two sequence-related genes of Drosophila melanogaster under reduced stringency hybridization conditions. The two kni homologous genes map close to kni in the proximal portion of the third chromosome. One of them is the previously identified gene knirps-related (knrl), kni and knrl are spatially co-regulated in both early and late stages of embryogenesis. Their posterior domains of expression at blastoderm stage are under the control of the maternal pattern organizer gene nanos. In contrast, the expression of the second kni homologous gene is restricted to the late embryonic gonads. Due to its site of expression, we termed this gene 'embryonic gonad' (egon). In addition to the conserved DNA-binding domain, these three genes share an additional sequence of 19 amino acids, the kni-box, adjacent to the finger region. The identical N-terminal Cys/Cys finger encoded by each of the three genes suggests that they code for DNA-binding proteins which might bind to similar (or even identical) target sequences.
缺口类分节基因的一个成员克尼普斯（kni）的假定指状结构域，被用于在较低严谨性杂交条件下分离黑腹果蝇的两个序列相关基因。这两个与kni同源的基因定位于第三条染色体近端靠近kni的位置。其中一个是先前已鉴定的基因克尼普斯相关基因（knrl），kni和knrl在胚胎发育的早期和晚期阶段在空间上共同受调控。它们在囊胚期的后部表达区域受母体模式组织者基因纳米斯的控制。相比之下，第二个与kni同源的基因的表达仅限于胚胎后期的性腺。由于其表达位点，我们将该基因命名为“胚胎性腺”（egon）。除了保守的DNA结合结构域外，这三个基因还共享一个由19个氨基酸组成的额外序列，即kni盒，毗邻指状区域。这三个基因各自编码的相同N端半胱氨酸/半胱氨酸指状结构表明，它们编码的是可能与相似（甚至相同）靶序列结合的DNA结合蛋白。
