Cupello A, Larsson S, Palm A, Rapallino M V
Centro di Neurofisiologia Cerebrale, C.N.R., Genova, Italy.
Neurochem Res. 1988 Jul;13(7):601-3. doi: 10.1007/BF00973274.
The reduction to the micro-scale of a recently described electrophoretic method for the evaluation of GABA catabolism by GABA-T is presented. The micromethod involves the electrophoresis, in 1 mm diam. capillaries, of small samples of mixtures of [14C]GABA and its metabolites. By coupling this procedure to previously devised micromethods, it was possible to evaluate GABA-T attack to 14C labeled GABA diffusing across a single microdissected neuronal membrane.
本文介绍了一种最近描述的用于评估GABA转氨酶（GABA-T）介导的GABA分解代谢的电泳方法在微尺度上的简化。该微方法涉及在直径为1毫米的毛细管中对[14C]GABA及其代谢物混合物的小样本进行电泳。通过将此程序与先前设计的微方法相结合，能够评估GABA-T对穿过单个显微解剖神经元膜扩散的14C标记GABA的作用。
