Cupello A, Rapallino M V, Besio G, Mainardi P
Anal Biochem. 1987 Jan;160(1):14-6. doi: 10.1016/0003-2697(87)90607-5.
An electrophoretic method for the separation of gamma-aminobutyric acid (GABA) from its metabolites after GABA-transaminase attack is presented. The method is based on the fact that at neutral pH GABA has no net electrical charge, whereas its major metabolites, succinic acid and Krebs cycle intermediates, are negatively charged. The method appears to be especially suitable for evaluation of true-labeled neurotransmitter within the radioactivity which is found in synaptosomes after labeled GABA-uptake studies.
本文介绍了一种电泳方法，用于在γ-氨基丁酸转氨酶攻击后从其代谢产物中分离γ-氨基丁酸（GABA）。该方法基于这样一个事实，即在中性pH值下，GABA没有净电荷，而其主要代谢产物琥珀酸和三羧酸循环中间体带负电荷。该方法似乎特别适用于评估在标记GABA摄取研究后在突触小体中发现的放射性内真正标记的神经递质。
