Jiangsu Key Laboratory of Oral Diseases, Department of Endodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Jiangsu Province Key Laboratory of Oral Diseases, Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, PR China.
Jiangsu Key Laboratory of Oral Diseases, Department of Prosthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Jiangsu Province Key Laboratory of Oral Diseases, Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, PR China.
J Mech Behav Biomed Mater. 2021 Sep;121:104610. doi: 10.1016/j.jmbbm.2021.104610. Epub 2021 Jun 1.
In the present study, we investigated the chemical interaction between 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP) and methacryloxypropyltrimethoxysilane (gamma-MPS) in one-bottle primer solutions and its effect on dentine bonding performance. Solutions containing 10 wt% 10-MDP and/or gamma-MPS at 0, 1, 5, 10, or 15 wt% were prepared, providing 10 experimental groups (labeled MDP/MPSxx or MPSxx, where MDP indicates the presence of 10 wt% MDP and xx is the wt% of gamma-MPS in the solution). Phosphoric-acid-etched dentine blocks were prepared from human molars and conditioned in the solutions before being used to build resin-dentine-bonded specimens, which were subsequently subjected to microtensile bond strength (μTBS) testing after 24-h or six-months water storage. Interfacial nanoleakage was evaluated by SEM observation. All the primer-conditioned samples showed significantly higher initial μTBS values than that of the control group, and six-months water storage significantly lowered the μTBS for all the groups; however, the decreases for MDP/MPS10 and MDP/MPS15 were significantly greater than those for MDP/MPS1 and the control solution. Furthermore, MDP/MPS10 and MDP/MPS15 groups also showed more serious nanoleakage. Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, and X-ray diffraction analyses were used to investigate the chemical affinity between 10-MDP and hydroxyapatite (HAp). In XRD analysis, the intensities of peaks assigned to 10-MDP-calcium salts were lower for the solutions containing gamma-MPS. Overall, the results indicate that the copresence of gamma-MPS (above 10 wt%) and 10-MDP in one-bottle primer solutions inhibit the formation of 10-MDP-calcium salts, leading to increased long-term nanoleakage and decreased bonding durability.
在本研究中，我们研究了 10-甲氧基癸基二氢磷酸酯（10-MDP）和甲氧基丙基三甲氧基硅烷（γ-MPS）在单瓶底涂剂溶液中的化学相互作用及其对牙本质粘结性能的影响。制备了含有 10wt% 10-MDP 和/或 γ-MPS 的 0、1、5、10 或 15wt%的溶液，提供了 10 个实验组（标记为 MDP/MPSxx 或 MPSxx，其中 MDP 表示存在 10wt%的 MDP，xx 是溶液中 γ-MPS 的重量百分比）。从人磨牙中制备磷酸酸蚀牙本质块，并在溶液中处理后用于构建树脂-牙本质粘结试件，随后在 24 小时或六个月水储存后进行微拉伸粘结强度（μTBS）测试。通过 SEM 观察评估界面纳米渗漏。所有底涂剂处理的样本初始 μTBS 值均明显高于对照组，六个月水储存后所有样本 μTBS 值均显著降低；然而，MDP/MPS10 和 MDP/MPS15 组的下降幅度明显大于 MDP/MPS1 和对照组。此外，MDP/MPS10 和 MDP/MPS15 组也表现出更严重的纳米渗漏。傅里叶变换红外光谱、X 射线光电子能谱和 X 射线衍射分析用于研究 10-MDP 和羟基磷灰石（HAp）之间的化学亲和力。在 XRD 分析中，含有 γ-MPS 的溶液中分配给 10-MDP-钙盐的峰强度较低。总体而言，结果表明，单瓶底涂剂溶液中γ-MPS（超过 10wt%）和 10-MDP 的共存抑制了 10-MDP-钙盐的形成，导致长期纳米渗漏增加和粘结耐久性降低。
