评价洗必泰和 MDP 的相互作用及其对牙本质粘结耐久性的影响。

Evaluation of the interaction of chlorhexidine and MDP and its effects on the durability of dentin bonding.

机构信息

Jiangsu Key Laboratory of Oral Diseases, Department of Endodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China.

Jiangsu Key Laboratory of Oral Diseases, Department of Prosthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China.

出版信息

Dent Mater. 2020 Dec;36(12):1624-1634. doi: 10.1016/j.dental.2020.10.006. Epub 2020 Nov 10.

DOI:10.1016/j.dental.2020.10.006

PMID:33183774

Abstract

OBJECTIVE

This study aimed to evaluate the potential interaction of chlorhexidine (CHX) and 10-methacryloyloxydecyl dihydrogen phosphate (MDP) and its effects on the durability of dentin bonding.

METHODS

Two commercial adhesives were tested: a MDP-free adhesive (Single Bond 2, SB2) and a MDP-containing adhesive (Single Bond Universal, SBU). Teeth were randomly assigned to six groups and tested for micro-tensile bond strength (μTBS): Ctr, direct bonding with SB2; CHX, CHX conditioning and SB2; MDP, MDP conditioning and SB2; CHX+MDP, combined CHX and MDP conditioning and SB2; SBU, direct bonding with SBU; CHX+SBU, CHX conditioning and SBU. The potential interaction of CHX and MDP was assessed by measuring nanoleakage, in situ zymography, and chemoanalytic characterization via Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and nuclear magnetic resonance (NMR). Specimens for μTBS and nanoleakage tests were first subjected to water storage for 24h or 6 months.

RESULTS

The initial μTBS values of the Ctr and CHX groups were significantly lower than those of the other four groups (P<0.05). Water storage for 6 months significantly weakened all groups (P<0.05), with the Ctr group showing the lowest μTBS. This group also showed more obvious nanoleakage than the other five groups. In situ zymography revealed that the Ctr group showed the strongest fluorescence and that the CHX+MDP group showed greater fluorescence than either CHX or MDP group. FTIR, XPS, and NMR indicated that MDP can interact with hydroxyapatite. NMR detected no Ca salt peak for MDP when it was combined with CHX.

SIGNIFICANCE

The application of either CHX or MDP alone can improve dentin bond durability. However, CHX may interfere with the formation of MDP-Ca salts.

摘要

目的

本研究旨在评估洗必泰和 10- 甲氧基二氢磷酸酯（MDP）的潜在相互作用及其对牙本质粘结耐久性的影响。

方法

本研究使用了两种商业性粘结剂：一种不含 MDP 的粘结剂（Single Bond 2，SB2）和一种含 MDP 的粘结剂（Single Bond Universal，SBU）。将牙齿随机分为六组，用于微拉伸粘结强度（μTBS）测试：Ctr，直接用 SB2 粘结；CHX，用 CHX 预处理后再用 SB2 粘结；MDP，用 MDP 预处理后再用 SB2 粘结；CHX+MDP，先用 CHX 和 MDP 预处理后再用 SB2 粘结；SBU，直接用 SBU 粘结；CHX+SBU，先用 CHX 预处理后再用 SBU 粘结。通过纳米渗漏测量、原位酶谱分析和傅里叶变换红外光谱（FTIR）、X 射线光电子能谱（XPS）和核磁共振（NMR）化学分析来评估 CHX 和 MDP 的潜在相互作用。用于 μTBS 和纳米渗漏测试的标本首先在水中储存 24 小时或 6 个月。

结果

Ctr 和 CHX 组的初始 μTBS 值明显低于其他四组（P<0.05）。6 个月的水储存显著降低了所有组的粘结强度（P<0.05），其中 Ctr 组的 μTBS 最低。该组也显示出比其他五组更明显的纳米渗漏。原位酶谱分析显示，Ctr 组显示出最强的荧光，而 CHX+MDP 组显示出比 CHX 或 MDP 组更强的荧光。FTIR、XPS 和 NMR 表明 MDP 可以与羟基磷灰石相互作用。当 MDP 与 CHX 结合时，NMR 未检测到 MDP 的钙盐峰。