Jiangsu Key Laboratory Sericutural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212100, China.
Food Funct. 2021 Jul 21;12(14):6240-6253. doi: 10.1039/d1fo00913c. Epub 2021 Jun 15.
To prepare a nutritional supplement using silkworm pupae oil (SPO) as a feedstock, a microfluidic reactor with a smart hydrogel immobilized lipase was first constructed to reduce the relative content of palmitic acid at sn-1,3 and improve the nutritional function. The effects of flow rate, reaction temperature, and substrate molar ratio were investigated. In vitro digestion and pH-stat models were employed to analyze the digestion feature after the modification of SPO, while HPLC-ELSD, zeta potential, DSC, and TGA were used to evaluate the nutritional function. The relative content of "OOO" and "OPO" type triglycerides was increased by 49.48% and 107.67%, and that of palmitic acid at sn-1,3 was decreased by 49.61% in 10 s. After the verification of the in vitro digestion model, the fatty acid release rate of the modified SPO was significantly improved by 22.07%, indicating the nutritional function improvement of SPO. Therefore, the nutritional function of SPO has been improved successfully by the application of a microchannel reactor with photo-immobilized lipase, which could set a reference for the utilization of insect oil resources.
为了制备以蚕蛹油（SPO）为原料的营养补充剂，首先构建了一种带有智能水凝胶固定化脂肪酶的微流反应器，以降低 sn-1,3 位的棕榈酸相对含量，提高营养价值。考察了流速、反应温度和底物摩尔比的影响。采用体外消化和 pH -stat 模型分析了 SPO 修饰后的消化特性，并用 HPLC-ELSD、Zeta 电位、DSC 和 TGA 评价了其营养价值。在 10 s 内，“OOO”和“OPO”型三酰甘油的相对含量分别增加了 49.48%和 107.67%，sn-1,3 位的棕榈酸含量降低了 49.61%。通过体外消化模型的验证，改性 SPO 的脂肪酸释放率提高了 22.07%，表明 SPO 的营养价值得到了提高。因此，通过应用带有光固定化脂肪酶的微通道反应器，成功提高了 SPO 的营养价值，为昆虫油脂资源的利用提供了参考。
