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多种核因子与尿激酶型纤溶酶原激活剂基因的启动子序列相互作用。

Multiple nuclear factors interact with promoter sequences of the urokinase-type plasminogen activator gene.

作者信息

von der Ahe D, Pearson D, Nakagawa J, Rajput B, Nagamine Y

机构信息

Friedrich Miescher-Institut, Basel, Switzerland.

出版信息

Nucleic Acids Res. 1988 Aug 11;16(15):7527-44. doi: 10.1093/nar/16.15.7527.

DOI:10.1093/nar/16.15.7527
PMID:3412894
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC338425/
Abstract

To characterize proteins that bind to the cyclic AMP inducible promoter of the urokinase-type plasminogen activator gene, we performed a DNAase I footprinting analysis. Within 500 nucleotides upstream of the transcription start site we found eight protected regions due to at least four different binding proteins. Among these is a single binding site for the transcription factor CTF/NF1, which is flanked on each side by two conserved binding sites for the transcription factor Sp1. A region at -380, which shares a similarity with sequences observed in the corresponding regions of other cyclic AMP regulated genes, was protected. This binding site contains a sequence of ten nucleotides which is repeated further upstream at -480 and also protected against DNAase I digestion. Comparisons of extracts from four different cell lines revealed that all DNA binding factors are present in nuclei of uPA expressing and nonexpressing cells. Mechanism underlying hormonal regulation of the gene is discussed.

摘要

为了鉴定与尿激酶型纤溶酶原激活剂基因的环磷酸腺苷诱导型启动子结合的蛋白质，我们进行了DNA酶I足迹分析。在转录起始位点上游500个核苷酸范围内，我们发现了由于至少四种不同结合蛋白而产生的八个受保护区域。其中有一个转录因子CTF/NF1的单一结合位点，其两侧各有两个转录因子Sp1的保守结合位点。一个位于-380的区域受到保护，该区域与其他环磷酸腺苷调节基因相应区域中观察到的序列相似。这个结合位点包含一个十核苷酸序列，该序列在-480处进一步上游重复，并且也能抵抗DNA酶I的消化。对四种不同细胞系提取物的比较显示，所有DNA结合因子都存在于表达和不表达uPA的细胞核中。本文讨论了该基因激素调节的潜在机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/338425/10eecc059ed6/nar00157-0320-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/338425/51ecc81ae97b/nar00157-0312-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/338425/d544918b3c26/nar00157-0313-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/338425/889d3033e71e/nar00157-0315-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/338425/f308fb4484af/nar00157-0317-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/338425/995c49f01a7a/nar00157-0318-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/338425/bb30e7007676/nar00157-0319-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/338425/10eecc059ed6/nar00157-0320-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/338425/51ecc81ae97b/nar00157-0312-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/338425/d544918b3c26/nar00157-0313-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/338425/889d3033e71e/nar00157-0315-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/338425/f308fb4484af/nar00157-0317-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/338425/995c49f01a7a/nar00157-0318-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/338425/bb30e7007676/nar00157-0319-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd96/338425/10eecc059ed6/nar00157-0320-a.jpg

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